Loading...
Reach Banner
Keywords
Last name
Institution

Christine Bear

TitleSenior Scientist
InstitutionThe Hospital for Sick Children
DepartmentMolecular Medicine
Address555 University Avenue
Toronto ON M5G 1X8
Phone416-813-1500
vCardDownload vCard

    Collapse Bibliographic 
    Collapse selected publications
    Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Faculty can login to make corrections and additions.
    List All   |   Timeline
    1. Wu YS, Jiang J, Ahmadi S, Lew A, Laselva O, Xia S, Bartlett C, Ip W, Wellhauser L, Ouyang H, Gonska T, Moraes TJ, Bear CE. ORKAMBI-Mediated Rescue of Mucociliary Clearance in Cystic Fibrosis Primary Respiratory Cultures Is Enhanced by Arginine Uptake, Arginase Inhibition, and Promotion of Nitric Oxide Signaling to the Cystic Fibrosis Transmembrane Conductance Regulator Channel. Mol Pharmacol. 2019 Oct; 96(4):515-525. PMID: 31427400.
      View in: PubMed
    2. Chin S, Ramjeesingh M, Hung M, Ereño-Oreba J, Cui H, Laselva O, Julien JP, Bear CE. Cholesterol Interaction Directly Enhances Intrinsic Activity of the Cystic Fibrosis Transmembrane Conductance Regulator (CFTR). Cells. 2019 Jul 31; 8(8). PMID: 31370288.
      View in: PubMed
    3. Wong AP, Shojaie S, Liang Q, Xia S, Di Paola M, Ahmadi S, Bilodeau C, Garner J, Post M, Duchesneau P, Waddell TK, Bear CE, Nagy A, Rossant J. Conversion of human and mouse fibroblasts into lung-like epithelial cells. Sci Rep. 2019 Jun 21; 9(1):9027. PMID: 31227724.
      View in: PubMed
    4. Ahmadi S, Wu YS, Li M, Ip W, Lloyd-Kuzik A, Di Paola M, Du K, Xia S, Lew A, Bozoky Z, Forman-Kay J, Bear CE, Gonska T. Augmentation of CFTR function in human bronchial epithelial cells via SLC6A14-dependent amino acid uptake: Implications for treatment of Cystic Fibrosis. Am J Respir Cell Mol Biol. 2019 Jun 12. PMID: 31189070.
      View in: PubMed
    5. Eckford PDW, McCormack J, Munsie L, He G, Stanojevic S, Pereira SL, Ho K, Avolio J, Bartlett C, Yang JY, Wong AP, Wellhauser L, Huan LJ, Jiang JX, Ouyang H, Du K, Klingel M, Kyriakopoulou L, Gonska T, Moraes TJ, Strug LJ, Rossant J, Ratjen F, Bear CE. The CF Canada-Sick Kids Program in individual CF therapy: A resource for the advancement of personalized medicine in CF. J Cyst Fibros. 2019 Jan; 18(1):35-43. PMID: 29685812.
      View in: PubMed
    6. Beaudoin T, Stone TA, Glibowicka M, Adams C, Yau Y, Ahmadi S, Bear CE, Grasemann H, Waters V, Deber CM. Activity of a novel antimicrobial peptide against Pseudomonas aeruginosa biofilms. Sci Rep. 2018 Oct 03; 8(1):14728. PMID: 30283025.
      View in: PubMed
    7. Ahmadi S, Xia S, Wu YS, Di Paola M, Kissoon R, Luk C, Lin F, Du K, Rommens J, Bear CE. SLC6A14, an amino acid transporter, modifies the primary CF defect in fluid secretion. Elife. 2018 07 13; 7. PMID: 30004386.
      View in: PubMed
    8. Laselva O, Marzaro G, Vaccarin C, Lampronti I, Tamanini A, Lippi G, Gambari R, Cabrini G, Bear CE, Chilin A, Dechecchi MC. Molecular Mechanism of Action of Trimethylangelicin Derivatives as CFTR Modulators. Front Pharmacol. 2018; 9:719. PMID: 30022950.
      View in: PubMed
    9. Chin S, Hung M, Won A, Wu YS, Ahmadi S, Yang D, Elmallah S, Toutah K, Hamilton CM, Young RN, Viirre RD, Yip CM, Bear CE. Lipophilicity of the Cystic Fibrosis Drug, Ivacaftor (VX-770), and Its Destabilizing Effect on the Major CF-causing Mutation: F508del. Mol Pharmacol. 2018 08; 94(2):917-925. PMID: 29903751.
      View in: PubMed
    10. Molinski SV, Shahani VM, Subramanian AS, MacKinnon SS, Woollard G, Laforet M, Laselva O, Morayniss LD, Bear CE, Windemuth A. Comprehensive mapping of cystic fibrosis mutations to CFTR protein identifies mutation clusters and molecular docking predicts corrector binding site. Proteins. 2018 08; 86(8):833-843. PMID: 29569753.
      View in: PubMed
    11. Laselva O, Molinski S, Casavola V, Bear CE. Correctors of the Major Cystic Fibrosis Mutant Interact through Membrane-Spanning Domains. Mol Pharmacol. 2018 06; 93(6):612-618. PMID: 29618585.
      View in: PubMed
    12. Cao H, Ouyang H, Grasemann H, Bartlett C, Du K, Duan R, Shi F, Estrada M, Seigel KE, Coates AL, Yeger H, Bear CE, Gonska T, Moraes TJ, Hu J. Transducing Airway Basal Cells with a Helper-Dependent Adenoviral Vector for Lung Gene Therapy. Hum Gene Ther. 2018 06; 29(6):643-652. PMID: 29320887.
      View in: PubMed
    13. Chang YH, Stone TA, Chin S, Glibowicka M, Bear CE, Deber CM. Structural effects of extracellular loop mutations in CFTR helical hairpins. Biochim Biophys Acta Biomembr. 2018 May; 1860(5):1092-1098. PMID: 29307731.
      View in: PubMed
    14. Di Paola M, Park AJ, Ahmadi S, Roach EJ, Wu YS, Struder-Kypke M, Lam JS, Bear CE, Khursigara CM. SLC6A14 Is a Genetic Modifier of Cystic Fibrosis That Regulates Pseudomonas aeruginosa Attachment to Human Bronchial Epithelial Cells. MBio. 2017 12 19; 8(6). PMID: 29259090.
      View in: PubMed
    15. Guo L, Karoubi G, Duchesneau P, Shutova MV, Sung HK, Tonge P, Bear C, Rogers I, Nagy A, Waddell TK. Generation of Induced Progenitor-like Cells from Mature Epithelial Cells Using Interrupted Reprogramming. Stem Cell Reports. 2017 12 12; 9(6):1780-1795. PMID: 29198829.
      View in: PubMed
    16. Molinski SV, Ahmadi S, Ip W, Ouyang H, Villella A, Miller JP, Lee PS, Kulleperuma K, Du K, Di Paola M, Eckford PD, Laselva O, Huan LJ, Wellhauser L, Li E, Ray PN, Pomès R, Moraes TJ, Gonska T, Ratjen F, Bear CE. Orkambi® and amplifier co-therapy improves function from a rare CFTR mutation in gene-edited cells and patient tissue. EMBO Mol Med. 2017 09; 9(9):1224-1243. PMID: 28667089.
      View in: PubMed
    17. Sheppard DN, Bear CE, de Jonge HR. Editorial overview: Respiratory: Transformational therapies for cystic fibrosis. Curr Opin Pharmacol. 2017 06; 34:viii-xi. PMID: 29221574.
      View in: PubMed
    18. Ahmadi S, Bozoky Z, Di Paola M, Xia S, Li C, Wong AP, Wellhauser L, Molinski SV, Ip W, Ouyang H, Avolio J, Forman-Kay JD, Ratjen F, Hirota JA, Rommens J, Rossant J, Gonska T, Moraes TJ, Bear CE. Phenotypic profiling of CFTR modulators in patient-derived respiratory epithelia. NPJ Genom Med. 2017 Apr 14; 2:12. PMID: 28649446.
      View in: PubMed
    19. Bozoky Z, Ahmadi S, Milman T, Kim TH, Du K, Di Paola M, Pasyk S, Pekhletski R, Keller JP, Bear CE, Forman-Kay JD. Synergy of cAMP and calcium signaling pathways in CFTR regulation. Proc Natl Acad Sci U S A. 2017 03 14; 114(11):E2086-E2095. PMID: 28242698.
      View in: PubMed
    20. Chin S, Yang D, Miles AJ, Eckford PD, Molinski S, Wallace BA, Bear CE. Attenuation of Phosphorylation-dependent Activation of Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) by Disease-causing Mutations at the Transmission Interface. J Biol Chem. 2017 02 03; 292(5):1988-1999. PMID: 28003367.
      View in: PubMed
    21. Strug LJ, Gonska T, He G, Keenan K, Ip W, Boëlle PY, Lin F, Panjwani N, Gong J, Li W, Soave D, Xiao B, Tullis E, Rabin H, Parkins MD, Price A, Zuberbuhler PC, Corvol H, Ratjen F, Sun L, Bear CE, Rommens JM. Cystic fibrosis gene modifier SLC26A9 modulates airway response to CFTR-directed therapeutics. Hum Mol Genet. 2016 10 15; 25(20):4590-4600. PMID: 28171547.
      View in: PubMed
    22. Chin S, Hung M, Bear CE. Current insights into the role of PKA phosphorylation in CFTR channel activity and the pharmacological rescue of cystic fibrosis disease-causing mutants. Cell Mol Life Sci. 2017 01; 74(1):57-66. PMID: 27722768.
      View in: PubMed
    23. Laselva O, Molinski S, Casavola V, Bear CE. The investigational Cystic Fibrosis drug Trimethylangelicin directly modulates CFTR by stabilizing the first membrane-spanning domain. Biochem Pharmacol. 2016 Nov 01; 119:85-92. PMID: 27614011.
      View in: PubMed
    24. Ehrhardt A, Chung WJ, Pyle LC, Wang W, Nowotarski K, Mulvihill CM, Ramjeesingh M, Hong J, Velu SE, Lewis HA, Atwell S, Aller S, Bear CE, Lukacs GL, Kirk KL, Sorscher EJ. Channel Gating Regulation by the Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) First Cytosolic Loop. J Biol Chem. 2016 Jan 22; 291(4):1854-65. PMID: 26627831.
      View in: PubMed
    25. Verkman AS, Edelman A, Amaral M, Mall MA, Beekman JM, Meiners T, Galietta LJ, Bear CE. Finding new drugs to enhance anion secretion in cystic fibrosis: Toward suitable systems for better drug screening. Report on the pre-conference meeting to the 12th ECFS Basic Science Conference, Albufeira, 25-28 March 2015. J Cyst Fibros. 2015 Nov; 14(6):700-5. PMID: 26474804.
      View in: PubMed
    26. Molinski SV, Ahmadi S, Hung M, Bear CE. Facilitating Structure-Function Studies of CFTR Modulator Sites with Efficiencies in Mutagenesis and Functional Screening. J Biomol Screen. 2015 Dec; 20(10):1204-17. PMID: 26385858.
      View in: PubMed
    27. Cao H, Ouyang H, Ip W, Du K, Duan W, Avolio J, Wu J, Duan C, Yeger H, Bear CE, Gonska T, Hu J, Moraes TJ. Testing gene therapy vectors in human primary nasal epithelial cultures. Mol Ther Methods Clin Dev. 2015; 2:15034. PMID: 26730394.
      View in: PubMed
    28. Ogawa M, Ogawa S, Bear CE, Ahmadi S, Chin S, Li B, Grompe M, Keller G, Kamath BM, Ghanekar A. Directed differentiation of cholangiocytes from human pluripotent stem cells. Nat Biotechnol. 2015 Aug; 33(8):853-61. PMID: 26167630.
      View in: PubMed
    29. Malik FA, Meissner A, Semenkov I, Molinski S, Pasyk S, Ahmadi S, Bui HH, Bear CE, Lidington D, Bolz SS. Sphingosine-1-Phosphate Is a Novel Regulator of Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) Activity. PLoS One. 2015; 10(6):e0130313. PMID: 26079370.
      View in: PubMed
    30. Eckford PD, Li C, Bear CE. Functional reconstitution and channel activity measurements of purified wildtype and mutant CFTR protein. J Vis Exp. 2015 Mar 09; (97). PMID: 25867140.
      View in: PubMed
    31. Wong AP, Chin S, Xia S, Garner J, Bear CE, Rossant J. Efficient generation of functional CFTR-expressing airway epithelial cells from human pluripotent stem cells. Nat Protoc. 2015 Mar; 10(3):363-81. PMID: 25654755.
      View in: PubMed
    32. Shojaie S, Ermini L, Ackerley C, Wang J, Chin S, Yeganeh B, Bilodeau M, Sambi M, Rogers I, Rossant J, Bear CE, Post M. Acellular lung scaffolds direct differentiation of endoderm to functional airway epithelial cells: requirement of matrix-bound HS proteoglycans. Stem Cell Reports. 2015 Mar 10; 4(3):419-30. PMID: 25660407.
      View in: PubMed
    33. Pasyk S, Molinski S, Ahmadi S, Ramjeesingh M, Huan LJ, Chin S, Du K, Yeger H, Taylor P, Moran MF, Bear CE. The major cystic fibrosis causing mutation exhibits defective propensity for phosphorylation. Proteomics. 2015 Jan; 15(2-3):447-61. PMID: 25330774.
      View in: PubMed
    34. Broadbent SD, Ramjeesingh M, Bear CE, Argent BE, Linsdell P, Gray MA. The cystic fibrosis transmembrane conductance regulator is an extracellular chloride sensor. Pflugers Arch. 2015 Aug; 467(8):1783-94. PMID: 25277268.
      View in: PubMed
    35. Jaecklin T, Duerr J, Huang H, Rafii M, Bear CE, Ratjen F, Pencharz P, Kavanagh BP, Mall MA, Grasemann H. Lung arginase expression and activity is increased in cystic fibrosis mouse models. J Appl Physiol (1985). 2014 Aug 01; 117(3):284-8. PMID: 24925982.
      View in: PubMed
    36. Eckford PD, Ramjeesingh M, Molinski S, Pasyk S, Dekkers JF, Li C, Ahmadi S, Ip W, Chung TE, Du K, Yeger H, Beekman J, Gonska T, Bear CE. VX-809 and related corrector compounds exhibit secondary activity stabilizing active F508del-CFTR after its partial rescue to the cell surface. Chem Biol. 2014 May 22; 21(5):666-78. PMID: 24726831.
      View in: PubMed
    37. Molinski SV, Gonska T, Huan LJ, Baskin B, Janahi IA, Ray PN, Bear CE. Genetic, cell biological, and clinical interrogation of the CFTR mutation c.3700 A>G (p.Ile1234Val) informs strategies for future medical intervention. Genet Med. 2014 Aug; 16(8):625-32. PMID: 24556927.
      View in: PubMed
    38. Islam ST, Eckford PD, Jones ML, Nugent T, Bear CE, Vogel C, Lam JS. Proton-dependent gating and proton uptake by Wzx support O-antigen-subunit antiport across the bacterial inner membrane. MBio. 2013 Sep 10; 4(5):e00678-13. PMID: 24023388.
      View in: PubMed
    39. D'Antonio C, Molinski S, Ahmadi S, Huan LJ, Wellhauser L, Bear CE. Conformational defects underlie proteasomal degradation of Dent's disease-causing mutants of ClC-5. Biochem J. 2013 Jun 15; 452(3):391-400. PMID: 23566014.
      View in: PubMed
    40. Bear CE. 50 years ago in the Journal of Pediatrics: the effect of N-acetylcysteine on the viscosity of tracheobronchial secretions in cystic fibrosis of the pancreas. J Pediatr. 2013 Jan; 162(1):85. PMID: 23260312.
      View in: PubMed
    41. Molinski S, Eckford PD, Pasyk S, Ahmadi S, Chin S, Bear CE. Functional Rescue of F508del-CFTR Using Small Molecule Correctors. Front Pharmacol. 2012; 3:160. PMID: 23055971.
      View in: PubMed
    42. Wong AP, Bear CE, Chin S, Pasceri P, Thompson TO, Huan LJ, Ratjen F, Ellis J, Rossant J. Directed differentiation of human pluripotent stem cells into mature airway epithelia expressing functional CFTR protein. Nat Biotechnol. 2012 Sep; 30(9):876-82. PMID: 22922672.
      View in: PubMed
    43. Eckford PD, Li C, Ramjeesingh M, Bear CE. Cystic fibrosis transmembrane conductance regulator (CFTR) potentiator VX-770 (ivacaftor) opens the defective channel gate of mutant CFTR in a phosphorylation-dependent but ATP-independent manner. J Biol Chem. 2012 Oct 26; 287(44):36639-49. PMID: 22942289.
      View in: PubMed
    44. Pasyk S, Molinski S, Yu W, Eckford PD, Bear CE. Identification and validation of hits from high throughput screens for CFTR modulators. Curr Pharm Des. 2012; 18(5):628-41. PMID: 22229556.
      View in: PubMed
    45. Alkhouri B, Denning RA, Kim Chiaw P, Eckford PD, Yu W, Li C, Bogojeski JJ, Bear CE, Viirre RD. Synthesis and properties of molecular probes for the rescue site on mutant cystic fibrosis transmembrane conductance regulator. J Med Chem. 2011 Dec 22; 54(24):8693-701. PMID: 22074181.
      View in: PubMed
    46. Kim Chiaw P, Eckford PD, Bear CE. Insights into the mechanisms underlying CFTR channel activity, the molecular basis for cystic fibrosis and strategies for therapy. Essays Biochem. 2011 Sep 07; 50(1):233-48. PMID: 21967060.
      View in: PubMed
    47. Whitney JC, Hay ID, Li C, Eckford PD, Robinson H, Amaya MF, Wood LF, Ohman DE, Bear CE, Rehm BH, Howell PL. Structural basis for alginate secretion across the bacterial outer membrane. Proc Natl Acad Sci U S A. 2011 Aug 09; 108(32):13083-8. PMID: 21778407.
      View in: PubMed
    48. Yu W, Kim Chiaw P, Bear CE. Probing conformational rescue induced by a chemical corrector of F508del-cystic fibrosis transmembrane conductance regulator (CFTR) mutant. J Biol Chem. 2011 Jul 15; 286(28):24714-25. PMID: 21602569.
      View in: PubMed
    49. Eckford PD, Bear CE. Targeting the regulation of CFTR channels. Biochem J. 2011 Apr 15; 435(2):e1-4. PMID: 21726198.
      View in: PubMed
    50. Wellhauser L, Luna-Chavez C, D'Antonio C, Tainer J, Bear CE. ATP induces conformational changes in the carboxyl-terminal region of ClC-5. J Biol Chem. 2011 Feb 25; 286(8):6733-41. PMID: 21173145.
      View in: PubMed
    51. Lamhonwah AM, Bear CE, Huan LJ, Kim Chiaw P, Ackerley CA, Tein I. Cystic fibrosis transmembrane conductance regulator in human muscle: Dysfunction causes abnormal metabolic recovery in exercise. Ann Neurol. 2010 Jun; 67(6):802-8. PMID: 20517942.
      View in: PubMed
    52. Kim Chiaw P, Wellhauser L, Huan LJ, Ramjeesingh M, Bear CE. A chemical corrector modifies the channel function of F508del-CFTR. Mol Pharmacol. 2010 Sep; 78(3):411-8. PMID: 20501743.
      View in: PubMed
    53. Cortez MA, Li C, Whitehead SN, Dhani SU, D'Antonio C, Huan LJ, Bennett SA, Snead OC, Bear CE. Disruption of ClC-2 expression is associated with progressive neurodegeneration in aging mice. Neuroscience. 2010 Apr 28; 167(1):154-62. PMID: 20116415.
      View in: PubMed
    54. Wellhauser L, D'Antonio C, Bear CE. ClC transporters: discoveries and challenges in defining the mechanisms underlying function and regulation of ClC-5. Pflugers Arch. 2010 Jul; 460(2):543-57. PMID: 20049483.
      View in: PubMed
    55. Kim Chiaw P, Huan LJ, Gagnon S, Ly D, Sweezey N, Rotin D, Deber CM, Bear CE. Functional rescue of DeltaF508-CFTR by peptides designed to mimic sorting motifs. Chem Biol. 2009 May 29; 16(5):520-30. PMID: 19477416.
      View in: PubMed
    56. Mohammad-Panah R, Wellhauser L, Steinberg BE, Wang Y, Huan LJ, Liu XD, Bear CE. An essential role for ClC-4 in transferrin receptor function revealed in studies of fibroblasts derived from Clcn4-null mice. J Cell Sci. 2009 Apr 15; 122(Pt 8):1229-37. PMID: 19339555.
      View in: PubMed
    57. Wellhauser L, Kim Chiaw P, Pasyk S, Li C, Ramjeesingh M, Bear CE. A small-molecule modulator interacts directly with deltaPhe508-CFTR to modify its ATPase activity and conformational stability. Mol Pharmacol. 2009 Jun; 75(6):1430-8. PMID: 19339490.
      View in: PubMed
    58. Cheung JC, Kim Chiaw P, Deber CM, Bear CE. A novel method for monitoring the cytosolic delivery of peptide cargo. J Control Release. 2009 Jul 01; 137(1):2-7. PMID: 19285529.
      View in: PubMed
    59. Pasyk S, Li C, Ramjeesingh M, Bear CE. Direct interaction of a small-molecule modulator with G551D-CFTR, a cystic fibrosis-causing mutation associated with severe disease. Biochem J. 2009 Feb 15; 418(1):185-90. PMID: 18945216.
      View in: PubMed
    60. Ramjeesingh M, Ugwu F, Stratford FL, Huan LJ, Li C, Bear CE. The intact CFTR protein mediates ATPase rather than adenylate kinase activity. Biochem J. 2008 Jun 01; 412(2):315-21. PMID: 18241200.
      View in: PubMed
    61. Cheung JC, Kim Chiaw P, Pasyk S, Bear CE. Molecular basis for the ATPase activity of CFTR. Arch Biochem Biophys. 2008 Aug 01; 476(1):95-100. PMID: 18417076.
      View in: PubMed
    62. Mumbengegwi DR, Li Q, Li C, Bear CE, Engelhardt JF. Evidence for a superoxide permeability pathway in endosomal membranes. Mol Cell Biol. 2008 Jun; 28(11):3700-12. PMID: 18378695.
      View in: PubMed
    63. Payandeh J, Li C, Ramjeesingh M, Poduch E, Bear CE, Pai EF. Probing structure-function relationships and gating mechanisms in the CorA Mg2+ transport system. J Biol Chem. 2008 Apr 25; 283(17):11721-33. PMID: 18276588.
      View in: PubMed
    64. Dhani SU, Kim Chiaw P, Huan LJ, Bear CE. ATP depletion inhibits the endocytosis of ClC-2. J Cell Physiol. 2008 Jan; 214(1):273-80. PMID: 17620322.
      View in: PubMed
    65. Stratford FL, Ramjeesingh M, Cheung JC, Huan LJ, Bear CE. The Walker B motif of the second nucleotide-binding domain (NBD2) of CFTR plays a key role in ATPase activity by the NBD1-NBD2 heterodimer. Biochem J. 2007 Jan 15; 401(2):581-6. PMID: 16989640.
      View in: PubMed
    66. Wellhauser L, Kuo HH, Stratford FL, Ramjeesingh M, Huan LJ, Luong W, Li C, Deber CM, Bear CE. Nucleotides bind to the C-terminus of ClC-5. Biochem J. 2006 Sep 01; 398(2):289-94. PMID: 16686597.
      View in: PubMed
    67. Ramjeesingh M, Li C, She YM, Bear CE. Evaluation of the membrane-spanning domain of ClC-2. Biochem J. 2006 Jun 15; 396(3):449-60. PMID: 16526942.
      View in: PubMed
    68. Dhani SU, Bear CE. Role of intramolecular and intermolecular interactions in ClC channel and transporter function. Pflugers Arch. 2006 Mar; 451(6):708-15. PMID: 16167151.
      View in: PubMed
    69. Benos DJ, Berdiev BK, Ismailov II, Ostedgaard LS, Kogan I, Li C, Ramjeesingh M, Bear CE. Methods to study CFTR protein in vitro. J Cyst Fibros. 2004 Aug; 3 Suppl 2:79-83. PMID: 15463933.
      View in: PubMed
    70. Sheppard DN, Gray MA, Gong X, Sohma Y, Kogan I, Benos DJ, Scott-Ward TS, Chen JH, Li H, Cai Z, Gupta J, Li C, Ramjeesingh M, Berdiev BK, Ismailov II, Bear CE, Hwang TC, Linsdell P, Hug MJ. The patch-clamp and planar lipid bilayer techniques: powerful and versatile tools to investigate the CFTR Cl- channel. J Cyst Fibros. 2004 Aug; 3 Suppl 2:101-8. PMID: 15463939.
      View in: PubMed
    71. Norez C, Heda GD, Jensen T, Kogan I, Hughes LK, Auzanneau C, Dérand R, Bulteau-Pignoux L, Li C, Ramjeesingh M, Li H, Sheppard DN, Bear CE, Riordan JR, Becq F. Determination of CFTR chloride channel activity and pharmacology using radiotracer flux methods. J Cyst Fibros. 2004 Aug; 3 Suppl 2:119-21. PMID: 15463942.
      View in: PubMed
    72. Kogan I, Ramjeesingh M, Bear CE. ATPase assay of purified, reconstituted CFTR protein. J Cyst Fibros. 2004 Aug; 3 Suppl 2:133-4. PMID: 15463945.
      View in: PubMed
    73. Kidd JF, Ramjeesingh M, Stratford F, Huan LJ, Bear CE. A heteromeric complex of the two nucleotide binding domains of cystic fibrosis transmembrane conductance regulator (CFTR) mediates ATPase activity. J Biol Chem. 2004 Oct 01; 279(40):41664-9. PMID: 15284228.
      View in: PubMed
    74. Kidd JF, Kogan I, Bear CE. Molecular basis for the chloride channel activity of cystic fibrosis transmembrane conductance regulator and the consequences of disease-causing mutations. Curr Top Dev Biol. 2004; 60:215-49. PMID: 15094300.
      View in: PubMed
    75. Grimard V, Li C, Ramjeesingh M, Bear CE, Goormaghtigh E, Ruysschaert JM. Phosphorylation-induced conformational changes of cystic fibrosis transmembrane conductance regulator monitored by attenuated total reflection-Fourier transform IR spectroscopy and fluorescence spectroscopy. J Biol Chem. 2004 Feb 13; 279(7):5528-36. PMID: 14660584.
      View in: PubMed
    76. Ramjeesingh M, Ugwu F, Li C, Dhani S, Huan LJ, Wang Y, Bear CE. Stable dimeric assembly of the second membrane-spanning domain of CFTR (cystic fibrosis transmembrane conductance regulator) reconstitutes a chloride-selective pore. Biochem J. 2003 Nov 01; 375(Pt 3):633-41. PMID: 12892562.
      View in: PubMed
    77. Ramjeesingh M, Kidd JF, Huan LJ, Wang Y, Bear CE. Dimeric cystic fibrosis transmembrane conductance regulator exists in the plasma membrane. Biochem J. 2003 Sep 15; 374(Pt 3):793-7. PMID: 12820897.
      View in: PubMed
    78. Mohammad-Panah R, Harrison R, Dhani S, Ackerley C, Huan LJ, Wang Y, Bear CE. The chloride channel ClC-4 contributes to endosomal acidification and trafficking. J Biol Chem. 2003 Aug 01; 278(31):29267-77. PMID: 12746443.
      View in: PubMed
    79. Kogan I, Ramjeesingh M, Li C, Kidd JF, Wang Y, Leslie EM, Cole SP, Bear CE. CFTR directly mediates nucleotide-regulated glutathione flux. EMBO J. 2003 May 01; 22(9):1981-9. PMID: 12727866.
      View in: PubMed
    80. Dhani SU, Mohammad-Panah R, Ahmed N, Ackerley C, Ramjeesingh M, Bear CE. Evidence for a functional interaction between the ClC-2 chloride channel and the retrograde motor dynein complex. J Biol Chem. 2003 May 02; 278(18):16262-70. PMID: 12601004.
      View in: PubMed
    81. Pan J, Bear C, Farragher S, Cutz E, Yeger H. Cystic fibrosis transmembrane conductance regulator modulates neurosecretory function in pulmonary neuroendocrine cell-related tumor cell line models. Am J Respir Cell Mol Biol. 2002 Nov; 27(5):553-60. PMID: 12397014.
      View in: PubMed
    82. Kidd JF, Bear CE. Epithelial cell chloride channel activity correlates with improved airway function in cystic fibrosis patients with the major mutant: Delta F508. Pediatr Res. 2002 Nov; 52(5):625-7. PMID: 12409505.
      View in: PubMed
    83. Kogan I, Ramjeesingh M, Li C, Bear CE. Studies of the molecular basis for cystic fibrosis using purified reconstituted CFTR protein. Methods Mol Med. 2002; 70:143-57. PMID: 11917519.
      View in: PubMed
    84. Mohammad-Panah R, Ackerley C, Rommens J, Choudhury M, Wang Y, Bear CE. The chloride channel ClC-4 co-localizes with cystic fibrosis transmembrane conductance regulator and may mediate chloride flux across the apical membrane of intestinal epithelia. J Biol Chem. 2002 Jan 04; 277(1):566-74. PMID: 11675385.
      View in: PubMed
    85. Ramjeesingh M, Li C, Kogan I, Wang Y, Huan LJ, Bear CE. A monomer is the minimum functional unit required for channel and ATPase activity of the cystic fibrosis transmembrane conductance regulator. Biochemistry. 2001 Sep 04; 40(35):10700-6. PMID: 11524016.
      View in: PubMed
    86. Yeger H, Pan J, Fu XW, Bear C, Cutz E. Expression of CFTR and Cl(-) conductances in cells of pulmonary neuroepithelial bodies. Am J Physiol Lung Cell Mol Physiol. 2001 Sep; 281(3):L713-21. PMID: 11504700.
      View in: PubMed
    87. Gyömörey K, Garami E, Galley K, Rommens JM, Bear CE. Non-CFTR chloride channels likely contribute to secretion in the murine small intestine. Pflugers Arch. 2001; 443 Suppl 1:S103-6. PMID: 11845313.
      View in: PubMed
    88. Kogan I, Ramjeesingh M, Huan LJ, Wang Y, Bear CE. Perturbation of the pore of the cystic fibrosis transmembrane conductance regulator (CFTR) inhibits its atpase activity. J Biol Chem. 2001 Apr 13; 276(15):11575-81. PMID: 11124965.
      View in: PubMed
    89. Ahmed N, Ramjeesingh M, Wong S, Varga A, Garami E, Bear CE. Chloride channel activity of ClC-2 is modified by the actin cytoskeleton. Biochem J. 2000 Dec 15; 352 Pt 3:789-94. PMID: 11104687.
      View in: PubMed
    90. Gyömörey K, Yeger H, Ackerley C, Garami E, Bear CE. Expression of the chloride channel ClC-2 in the murine small intestine epithelium. Am J Physiol Cell Physiol. 2000 Dec; 279(6):C1787-94. PMID: 11078693.
      View in: PubMed
    91. Gyömörey K, Rozmahel R, Bear CE. Amelioration of intestinal disease severity in cystic fibrosis mice is associated with improved chloride secretory capacity. Pediatr Res. 2000 Dec; 48(6):731-4. PMID: 11102538.
      View in: PubMed
    92. Mohammad-Panah R, Gyomorey K, Rommens J, Choudhury M, Li C, Wang Y, Bear CE. ClC-2 contributes to native chloride secretion by a human intestinal cell line, Caco-2. J Biol Chem. 2001 Mar 16; 276(11):8306-13. PMID: 11096079.
      View in: PubMed
    93. Ramjeesingh M, Li C, Huan LJ, Garami E, Wang Y, Bear CE. Quaternary structure of the chloride channel ClC-2. Biochemistry. 2000 Nov 14; 39(45):13838-47. PMID: 11076524.
      View in: PubMed
    94. Ramjeesingh M, Huan LJ, Garami E, Bear CE. Novel method for evaluation of the oligomeric structure of membrane proteins. Biochem J. 1999 Aug 15; 342 ( Pt 1):119-23. PMID: 10432308.
      View in: PubMed
    95. Ramjeesingh M, Li C, Garami E, Huan LJ, Galley K, Wang Y, Bear CE. Walker mutations reveal loose relationship between catalytic and channel-gating activities of purified CFTR (cystic fibrosis transmembrane conductance regulator). Biochemistry. 1999 Feb 02; 38(5):1463-8. PMID: 9931011.
      View in: PubMed
    96. Xiong H, Li C, Garami E, Wang Y, Ramjeesingh M, Galley K, Bear CE. ClC-2 activation modulates regulatory volume decrease. J Membr Biol. 1999 Feb 01; 167(3):215-21. PMID: 9929373.
      View in: PubMed
    97. Ramjeesingh M, Garami E, Galley K, Li C, Wang Y, Bear CE. Purification and reconstitution of epithelial chloride channel cystic fibrosis transmembrane conductance regulator. Methods Enzymol. 1999; 294:227-46. PMID: 9916230.
      View in: PubMed
    98. Li C, Breton S, Morrison R, Cannon CL, Emma F, Sanchez-Olea R, Bear C, Strange K. Recombinant pICln forms highly cation-selective channels when reconstituted into artificial and biological membranes. J Gen Physiol. 1998 Dec; 112(6):727-36. PMID: 9834142.
      View in: PubMed
    99. Pasyk EA, Morin XK, Zeman P, Garami E, Galley K, Huan LJ, Wang Y, Bear CE. A conserved region of the R domain of cystic fibrosis transmembrane conductance regulator is important in processing and function. J Biol Chem. 1998 Nov 27; 273(48):31759-64. PMID: 9822639.
      View in: PubMed
    100. Ramjeesingh M, Huan LJ, Wilschanski M, Durie P, Li C, Gyomorey K, Wang Y, Kent G, Tanswell KA, Cutz E, Ackerley C, Bear CE. Assessment of the efficacy of in vivo CFTR protein replacement therapy in CF mice. Hum Gene Ther. 1998 Mar 01; 9(4):521-8. PMID: 9525313.
      View in: PubMed
    101. Kent G, Iles R, Bear CE, Huan LJ, Griesenbach U, McKerlie C, Frndova H, Ackerley C, Gosselin D, Radzioch D, O'Brodovich H, Tsui LC, Buchwald M, Tanswell AK. Lung disease in mice with cystic fibrosis. J Clin Invest. 1997 Dec 15; 100(12):3060-9. PMID: 9399953.
      View in: PubMed
    102. Bear CE, Li C, Galley K, Wang Y, Garami E, Ramjeesingh M. Coupling of ATP hydrolysis with channel gating by purified, reconstituted CFTR. J Bioenerg Biomembr. 1997 Oct; 29(5):465-73. PMID: 9511931.
      View in: PubMed
    103. Ramjeesingh M, Li C, Garami E, Huan LJ, Hewryk M, Wang Y, Galley K, Bear CE. A novel procedure for the efficient purification of the cystic fibrosis transmembrane conductance regulator (CFTR). Biochem J. 1997 Oct 01; 327 ( Pt 1):17-21. PMID: 9355728.
      View in: PubMed
    104. Rozmahel R, Gyömörey K, Plyte S, Nguyen V, Wilschanski M, Durie P, Bear CE, Tsui LC. Incomplete rescue of cystic fibrosis transmembrane conductance regulator deficient mice by the human CFTR cDNA. Hum Mol Genet. 1997 Jul; 6(7):1153-62. PMID: 9215687.
      View in: PubMed
    105. Li C, Ramjeesingh M, Wang W, Garami E, Hewryk M, Lee D, Rommens JM, Galley K, Bear CE. ATPase activity of the cystic fibrosis transmembrane conductance regulator. J Biol Chem. 1996 Nov 08; 271(45):28463-8. PMID: 8910473.
      View in: PubMed
    106. Li C, Ramjeesingh M, Bear CE. Purified cystic fibrosis transmembrane conductance regulator (CFTR) does not function as an ATP channel. J Biol Chem. 1996 May 17; 271(20):11623-6. PMID: 8662751.
      View in: PubMed
    107. Rozmahel R, Wilschanski M, Matin A, Plyte S, Oliver M, Auerbach W, Moore A, Forstner J, Durie P, Nadeau J, Bear C, Tsui LC. Modulation of disease severity in cystic fibrosis transmembrane conductance regulator deficient mice by a secondary genetic factor. Nat Genet. 1996 Mar; 12(3):280-7. PMID: 8589719.
      View in: PubMed
    108. Wilschanski MA, Rozmahel R, Beharry S, Kent G, Li C, Tsui LC, Durie P, Bear CE. In vivo measurements of ion transport in long-living CF mice. Biochem Biophys Res Commun. 1996 Feb 27; 219(3):753-9. PMID: 8645253.
      View in: PubMed
    109. Morin XK, Bond TD, Loo TW, Clarke DM, Bear CE. Failure of P-glycoprotein (MDR1) expressed in Xenopus oocytes to produce swelling-activated chloride channel activity. J Physiol. 1995 Aug 01; 486 ( Pt 3):707-14. PMID: 7473231.
      View in: PubMed
    110. O'Riordan CR, Erickson A, Bear C, Li C, Manavalan P, Wang KX, Marshall J, Scheule RK, McPherson JM, Cheng SH, et al. Purification and characterization of recombinant cystic fibrosis transmembrane conductance regulator from Chinese hamster ovary and insect cells. J Biol Chem. 1995 Jul 14; 270(28):17033-43. PMID: 7542655.
      View in: PubMed
    111. Krolczyk AJ, Bear CE, Lai PF, Schimmer BP. Effects of mutations in cAMP-dependent protein kinase on chloride efflux in Caco-2 human colonic carcinoma cells. J Cell Physiol. 1995 Jan; 162(1):64-73. PMID: 7529238.
      View in: PubMed
    112. Bear CE. Drugs transported by P-glycoprotein inhibit a 40 pS outwardly rectifying chloride channel. Biochem Biophys Res Commun. 1994 Apr 15; 200(1):513-21. PMID: 7909435.
      View in: PubMed
    113. Lukacs GL, Chang XB, Bear C, Kartner N, Mohamed A, Riordan JR, Grinstein S. The delta F508 mutation decreases the stability of cystic fibrosis transmembrane conductance regulator in the plasma membrane. Determination of functional half-lives on transfected cells. J Biol Chem. 1993 Oct 15; 268(29):21592-8. PMID: 7691813.
      View in: PubMed
    114. O'Brodovich H, Wang X, Li C, Rafii B, Correa J, Bear C. Novobiocin forms cation-permeable ion channels in rat fetal distal lung epithelium. Am J Physiol. 1993 Jun; 264(6 Pt 1):C1532-7. PMID: 7687410.
      View in: PubMed
    115. Li C, Ramjeesingh M, Reyes E, Jensen T, Chang X, Rommens JM, Bear CE. The cystic fibrosis mutation (delta F508) does not influence the chloride channel activity of CFTR. Nat Genet. 1993 Apr; 3(4):311-6. PMID: 7526932.
      View in: PubMed
    116. Bear C, Ling V. Multidrug resistance and cystic fibrosis genes: complementarity of function? Trends Genet. 1993 Mar; 9(3):67-8. PMID: 7683829.
      View in: PubMed
    117. Sood R, Bear C, Auerbach W, Reyes E, Jensen T, Kartner N, Riordan JR, Buchwald M. Regulation of CFTR expression and function during differentiation of intestinal epithelial cells. EMBO J. 1992 Jul; 11(7):2487-94. PMID: 1378393.
      View in: PubMed
    118. Bear CE, Li CH, Kartner N, Bridges RJ, Jensen TJ, Ramjeesingh M, Riordan JR. Purification and functional reconstitution of the cystic fibrosis transmembrane conductance regulator (CFTR). Cell. 1992 Feb 21; 68(4):809-18. PMID: 1371239.
      View in: PubMed
    119. Bear CE, Reyes EF. cAMP-activated chloride conductance in the colonic cell line, Caco-2. Am J Physiol. 1992 Jan; 262(1 Pt 1):C251-6. PMID: 1370744.
      View in: PubMed
    120. Bear CE, Li CH. Calcium-permeable channels in rat hepatoma cells are activated by extracellular nucleotides. Am J Physiol. 1991 Dec; 261(6 Pt 1):C1018-24. PMID: 1662901.
      View in: PubMed
    121. Bear CE. A K(+)-selective channel in the colonic carcinoma cell line: CaCo-2 is activated with membrane stretch. Biochim Biophys Acta. 1991 Nov 04; 1069(2):267-72. PMID: 1932066.
      View in: PubMed
    122. Bear CE, Duguay F, Naismith AL, Kartner N, Hanrahan JW, Riordan JR. Cl- channel activity in Xenopus oocytes expressing the cystic fibrosis gene. J Biol Chem. 1991 Oct 15; 266(29):19142-5. PMID: 1717461.
      View in: PubMed
    123. Rommens JM, Dho S, Bear CE, Kartner N, Kennedy D, Riordan JR, Tsui LC, Foskett JK. cAMP-inducible chloride conductance in mouse fibroblast lines stably expressing the human cystic fibrosis transmembrane conductance regulator. Proc Natl Acad Sci U S A. 1991 Sep 01; 88(17):7500-4. PMID: 1715567.
      View in: PubMed
    124. Kartner N, Hanrahan JW, Jensen TJ, Naismith AL, Sun SZ, Ackerley CA, Reyes EF, Tsui LC, Rommens JM, Bear CE, et al. Expression of the cystic fibrosis gene in non-epithelial invertebrate cells produces a regulated anion conductance. Cell. 1991 Feb 22; 64(4):681-91. PMID: 1705179.
      View in: PubMed
    125. Bear CE. Regulation of ion conductance in human skin fibroblasts. Adv Exp Med Biol. 1991; 290:273-83; discussion 283-5. PMID: 1719764.
      View in: PubMed
    126. Bear CE. A nonselective cation channel in rat liver cells is activated by membrane stretch. Am J Physiol. 1990 Mar; 258(3 Pt 1):C421-8. PMID: 1690515.
      View in: PubMed
    127. Rothstein A, Bear C. Cell volume changes and the activity of the chloride conductance path. Ann N Y Acad Sci. 1989; 574:294-308. PMID: 2561323.
      View in: PubMed
    128. Bear CE, Davison JS, Shaffer EA. Intracellular pH influences the resting membrane potential of isolated rat hepatocytes. Biochim Biophys Acta. 1988 Oct 06; 944(2):113-20. PMID: 3179283.
      View in: PubMed
    129. Bear CE. Phosphorylation-activated chloride channels in human skin fibroblasts. FEBS Lett. 1988 Sep 12; 237(1-2):145-9. PMID: 2458964.
      View in: PubMed
    130. Bear CE, Davison JS, Shaffer EA. Sodium-dependent taurocholate uptake by isolated rat hepatocytes occurs through an electrogenic mechanism. Biochim Biophys Acta. 1987 Oct 02; 903(2):388-94. PMID: 2443174.
      View in: PubMed
    131. Bear CE, Petersen OH. L-alanine evokes opening of single Ca2+-activated K+ channels in rat liver cells. Pflugers Arch. 1987 Oct; 410(3):342-4. PMID: 2446259.
      View in: PubMed
    132. Minuk GY, Bear CE, Sarjeant EJ. Sodium-independent, bicuculline-sensitive [3H]GABA binding to isolated rat hepatocytes. Am J Physiol. 1987 May; 252(5 Pt 1):G642-7. PMID: 3578523.
      View in: PubMed
    133. Bear C, Shaffer EA, Davison JS. The electrogenic effect of sodium taurocholate on rat hepatocyte couplets. Proc West Pharmacol Soc. 1987; 30:109-11. PMID: 3628259.
      View in: PubMed
    134. Bear CE, Petrunka CN, Strasberg SM. Evidence for a channel for the electrogenic transport of chloride ion in the rat hepatocyte. Hepatology. 1985 May-Jun; 5(3):383-91. PMID: 2581880.
      View in: PubMed
    Bear's networks
    Click the "See All" links for more information and interactive visualizations!
    Concepts Expand Description
    _
    Co-Authors Expand Description
    _
    Similar People Expand Description
    _
    Same Department Expand Description