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Lynne Howell

TitleSenior Scientist
InstitutionThe Hospital for Sick Children
DepartmentMolecular Medicine
Address555 University Avenue
Toronto ON M5G 1X8
Phone416-813-1500
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    Collapse Bibliographic 
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    Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Faculty can login to make corrections and additions.
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    1. Ostapska H, Howell PL, Sheppard DC. Deacetylated microbial biofilm exopolysaccharides: It pays to be positive. PLoS Pathog. 2018 Dec; 14(12):e1007411. PMID: 30589915.
      View in: PubMed
    2. Little DJ, Pfoh R, Le Mauff F, Bamford NC, Notte C, Baker P, Guragain M, Robinson H, Pier GB, Nitz M, Deora R, Sheppard DC, Howell PL. PgaB orthologues contain a glycoside hydrolase domain that cleaves deacetylated poly-ß(1,6)-N-acetylglucosamine and can disrupt bacterial biofilms. PLoS Pathog. 2018 04; 14(4):e1006998. PMID: 29684093.
      View in: PubMed
    3. Sychantha D, Chapman RN, Bamford NC, Boons GJ, Howell PL, Clarke AJ. Molecular Basis for the Attachment of S-Layer Proteins to the Cell Wall of Bacillus anthracis. Biochemistry. 2018 04 03; 57(13):1949-1953. PMID: 29522326.
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    4. Asker D, Awad TS, Baker P, Howell PL, Hatton BD. Non-eluting, surface-bound enzymes disrupt surface attachment of bacteria by continuous biofilm polysaccharide degradation. Biomaterials. 2018 Jun; 167:168-176. PMID: 29571052.
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    5. Snarr BD, Howell PL, Sheppard DC. Hoisted by their own petard: do microbial enzymes hold the solution to treating and preventing biofilm infections? Future Microbiol. 2018 03; 13:395-398. PMID: 29441819.
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    6. Sychantha D, Little DJ, Chapman RN, Boons GJ, Robinson H, Howell PL, Clarke AJ. PatB1 is an O-acetyltransferase that decorates secondary cell wall polysaccharides. Nat Chem Biol. 2018 Jan; 14(1):79-85. PMID: 29083419.
      View in: PubMed
    7. Sychantha D, Jones CS, Little DJ, Moynihan PJ, Robinson H, Galley NF, Roper DI, Dowson CG, Howell PL, Clarke AJ. In vitro characterization of the antivirulence target of Gram-positive pathogens, peptidoglycan O-acetyltransferase A (OatA). PLoS Pathog. 2017 Oct; 13(10):e1006667. PMID: 29077761.
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    8. Marmont LS, Whitfield GB, Rich JD, Yip P, Giesbrecht LB, Stremick CA, Whitney JC, Parsek MR, Harrison JJ, Howell PL. PelA and PelB proteins form a modification and secretion complex essential for Pel polysaccharide-dependent biofilm formation in Pseudomonas aeruginosa. J Biol Chem. 2017 11 24; 292(47):19411-19422. PMID: 28972168.
      View in: PubMed
    9. Zhang Q, Howell PL, Overkleeft HS, Filippov DV, van der Marel GA, Codée JDC. Chemical synthesis of guanosine diphosphate mannuronic acid (GDP-ManA) and its C-4-O-methyl and C-4-deoxy congeners. Carbohydr Res. 2017 Oct 10; 450:12-18. PMID: 28822279.
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    10. Buensuceso RNC, Daniel-Ivad M, Kilmury SLN, Leighton TL, Harvey H, Howell PL, Burrows LL. Cyclic AMP-Independent Control of Twitching Motility in Pseudomonas aeruginosa. J Bacteriol. 2017 08 15; 199(16). PMID: 28583947.
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    11. Snarr BD, Baker P, Bamford NC, Sato Y, Liu H, Lehoux M, Gravelat FN, Ostapska H, Baistrocchi SR, Cerone RP, Filler EE, Parsek MR, Filler SG, Howell PL, Sheppard DC. Microbial glycoside hydrolases as antibiofilm agents with cross-kingdom activity. Proc Natl Acad Sci U S A. 2017 07 03; 114(27):7124-7129. PMID: 28634301.
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    12. McCallum M, Tammam S, Khan A, Burrows LL, Howell PL. The molecular mechanism of the type IVa pilus motors. Nat Commun. 2017 05 05; 8:15091. PMID: 28474682.
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    13. Limoli DH, Whitfield GB, Kitao T, Ivey ML, Davis MR, Grahl N, Hogan DA, Rahme LG, Howell PL, O'Toole GA, Goldberg JB. Pseudomonas aeruginosa Alginate Overproduction Promotes Coexistence with Staphylococcus aureus in a Model of Cystic Fibrosis Respiratory Infection. MBio. 2017 03 21; 8(2). PMID: 28325763.
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    14. Marmont LS, Rich JD, Whitney JC, Whitfield GB, Almblad H, Robinson H, Parsek MR, Harrison JJ, Howell PL. Oligomeric lipoprotein PelC guides Pel polysaccharide export across the outer membrane of Pseudomonas aeruginosa. Proc Natl Acad Sci U S A. 2017 03 14; 114(11):2892-2897. PMID: 28242707.
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    15. Carter T, Buensuceso RN, Tammam S, Lamers RP, Harvey H, Howell PL, Burrows LL. The Type IVa Pilus Machinery Is Recruited to Sites of Future Cell Division. MBio. 2017 01 31; 8(1). PMID: 28143978.
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    16. Koo J, Lamers RP, Rubinstein JL, Burrows LL, Howell PL. Structure of the Pseudomonas aeruginosa Type IVa Pilus Secretin at 7.4 Å. Structure. 2016 Oct 04; 24(10):1778-1787. PMID: 27705815.
      View in: PubMed
    17. Leighton TL, Yong DH, Howell PL, Burrows LL. Type IV Pilus Alignment Subcomplex Proteins PilN and PilO Form Homo- and Heterodimers in Vivo. J Biol Chem. 2016 09 16; 291(38):19923-38. PMID: 27474743.
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    18. Buensuceso RN, Nguyen Y, Zhang K, Daniel-Ivad M, Sugiman-Marangos SN, Fleetwood AD, Zhulin IB, Junop MS, Howell PL, Burrows LL. The Conserved Tetratricopeptide Repeat-Containing C-Terminal Domain of Pseudomonas aeruginosa FimV Is Required for Its Cyclic AMP-Dependent and -Independent Functions. J Bacteriol. 2016 08 15; 198(16):2263-74. PMID: 27297880.
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    19. Baker P, Hill PJ, Snarr BD, Alnabelseya N, Pestrak MJ, Lee MJ, Jennings LK, Tam J, Melnyk RA, Parsek MR, Sheppard DC, Wozniak DJ, Howell PL. Exopolysaccharide biosynthetic glycoside hydrolases can be utilized to disrupt and prevent Pseudomonas aeruginosa biofilms. Sci Adv. 2016 05; 2(5):e1501632. PMID: 27386527.
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    20. Sheppard DC, Howell PL. Biofilm Exopolysaccharides of Pathogenic Fungi: Lessons from Bacteria. J Biol Chem. 2016 Jun 10; 291(24):12529-37. PMID: 27129222.
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    21. Lee MJ, Geller AM, Bamford NC, Liu H, Gravelat FN, Snarr BD, Le Mauff F, Chabot J, Ralph B, Ostapska H, Lehoux M, Cerone RP, Baptista SD, Vinogradov E, Stajich JE, Filler SG, Howell PL, Sheppard DC. Deacetylation of Fungal Exopolysaccharide Mediates Adhesion and Biofilm Formation. MBio. 2016 Apr 05; 7(2):e00252-16. PMID: 27048799.
      View in: PubMed
    22. McCallum M, Tammam S, Little DJ, Robinson H, Koo J, Shah M, Calmettes C, Moraes TF, Burrows LL, Howell PL. PilN Binding Modulates the Structure and Binding Partners of the Pseudomonas aeruginosa Type IVa Pilus Protein PilM. J Biol Chem. 2016 May 20; 291(21):11003-15. PMID: 27022027.
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    23. Hmelo LR, Borlee BR, Almblad H, Love ME, Randall TE, Tseng BS, Lin C, Irie Y, Storek KM, Yang JJ, Siehnel RJ, Howell PL, Singh PK, Tolker-Nielsen T, Parsek MR, Schweizer HP, Harrison JJ. Precision-engineering the Pseudomonas aeruginosa genome with two-step allelic exchange. Nat Protoc. 2015 Nov; 10(11):1820-41. PMID: 26492139; PMCID: PMC4862005.
    24. Baker P, Whitfield GB, Hill PJ, Little DJ, Pestrak MJ, Robinson H, Wozniak DJ, Howell PL. Characterization of the Pseudomonas aeruginosa Glycoside Hydrolase PslG Reveals That Its Levels Are Critical for Psl Polysaccharide Biosynthesis and Biofilm Formation. J Biol Chem. 2015 Nov 20; 290(47):28374-87. PMID: 26424791.
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    25. Bamford NC, Snarr BD, Gravelat FN, Little DJ, Lee MJ, Zacharias CA, Chabot JC, Geller AM, Baptista SD, Baker P, Robinson H, Howell PL, Sheppard DC. Sph3 Is a Glycoside Hydrolase Required for the Biosynthesis of Galactosaminogalactan in Aspergillus fumigatus. J Biol Chem. 2015 Nov 13; 290(46):27438-50. PMID: 26342082.
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    26. Jennings LK, Storek KM, Ledvina HE, Coulon C, Marmont LS, Sadovskaya I, Secor PR, Tseng BS, Scian M, Filloux A, Wozniak DJ, Howell PL, Parsek MR. Pel is a cationic exopolysaccharide that cross-links extracellular DNA in the Pseudomonas aeruginosa biofilm matrix. Proc Natl Acad Sci U S A. 2015 Sep 08; 112(36):11353-8. PMID: 26311845; PMCID: PMC4568648.
    27. Little DJ, Milek S, Bamford NC, Ganguly T, DiFrancesco BR, Nitz M, Deora R, Howell PL. The protein BpsB is a poly-ß-1,6-N-acetyl-D-glucosamine deacetylase required for biofilm formation in Bordetella bronchiseptica. J Biol Chem. 2015 Sep 11; 290(37):22827-40. PMID: 26203190.
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    28. Leighton TL, Buensuceso RN, Howell PL, Burrows LL. Biogenesis of Pseudomonas aeruginosa type IV pili and regulation of their function. Environ Microbiol. 2015 Nov; 17(11):4148-63. PMID: 25808785.
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    29. Roux D, Cywes-Bentley C, Zhang YF, Pons S, Konkol M, Kearns DB, Little DJ, Howell PL, Skurnik D, Pier GB. Identification of Poly-N-acetylglucosamine as a Major Polysaccharide Component of the Bacillus subtilis Biofilm Matrix. J Biol Chem. 2015 Jul 31; 290(31):19261-72. PMID: 26078454; PMCID: PMC4521046.
    30. Ariyakumaran R, Pokrovskaya V, Little DJ, Howell PL, Nitz M. Direct Staudinger-Phosphonite Reaction Provides Methylphosphonamidates as Inhibitors of CE4 De-N-acetylases. Chembiochem. 2015 Jun 15; 16(9):1350-6. PMID: 25864869.
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    31. Whitfield GB, Marmont LS, Howell PL. Enzymatic modifications of exopolysaccharides enhance bacterial persistence. Front Microbiol. 2015; 6:471. PMID: 26029200; PMCID: PMC4432689.
    32. Leighton TL, Dayalani N, Sampaleanu LM, Howell PL, Burrows LL. Novel Role for PilNO in Type IV Pilus Retraction Revealed by Alignment Subcomplex Mutations. J Bacteriol. 2015 Jul; 197(13):2229-2238. PMID: 25917913.
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    33. Whitney JC, Whitfield GB, Marmont LS, Yip P, Neculai AM, Lobsanov YD, Robinson H, Ohman DE, Howell PL. Dimeric c-di-GMP is required for post-translational regulation of alginate production in Pseudomonas aeruginosa. J Biol Chem. 2015 May 15; 290(20):12451-62. PMID: 25817996; PMCID: PMC4432266.
    34. Little DJ, Bamford NC, Pokrovskaya V, Robinson H, Nitz M, Howell PL. Structural basis for the De-N-acetylation of Poly-ß-1,6-N-acetyl-D-glucosamine in Gram-positive bacteria. J Biol Chem. 2014 Dec 26; 289(52):35907-17. PMID: 25359777.
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    35. Baker P, Ricer T, Moynihan PJ, Kitova EN, Walvoort MT, Little DJ, Whitney JC, Dawson K, Weadge JT, Robinson H, Ohman DE, Codée JD, Klassen JS, Clarke AJ, Howell PL. P. aeruginosa SGNH hydrolase-like proteins AlgJ and AlgX have similar topology but separate and distinct roles in alginate acetylation. PLoS Pathog. 2014 Aug; 10(8):e1004334. PMID: 25165982; PMCID: PMC4148444.
    36. Tan J, Rouse SL, Li D, Pye VE, Vogeley L, Brinth AR, El Arnaout T, Whitney JC, Howell PL, Sansom MS, Caffrey M. A conformational landscape for alginate secretion across the outer membrane of Pseudomonas aeruginosa. Acta Crystallogr D Biol Crystallogr. 2014 Aug; 70(Pt 8):2054-68. PMID: 25084326; PMCID: PMC4118822.
    37. Little DJ, Li G, Ing C, DiFrancesco BR, Bamford NC, Robinson H, Nitz M, Pomès R, Howell PL. Modification and periplasmic translocation of the biofilm exopolysaccharide poly-ß-1,6-N-acetyl-D-glucosamine. Proc Natl Acad Sci U S A. 2014 Jul 29; 111(30):11013-8. PMID: 24994902; PMCID: PMC4121833.
    38. Wolfram F, Kitova EN, Robinson H, Walvoort MT, Codée JD, Klassen JS, Howell PL. Catalytic mechanism and mode of action of the periplasmic alginate epimerase AlgG. J Biol Chem. 2014 Feb 28; 289(9):6006-19. PMID: 24398681.
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    39. Pokrovskaya V, Poloczek J, Little DJ, Griffiths H, Howell PL, Nitz M. Functional characterization of Staphylococcus epidermidis IcaB, a de-N-acetylase important for biofilm formation. Biochemistry. 2013 Aug 13; 52(32):5463-71. PMID: 23866051.
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    40. Riley LM, Weadge JT, Baker P, Robinson H, Codée JD, Tipton PA, Ohman DE, Howell PL. Structural and functional characterization of Pseudomonas aeruginosa AlgX: role of AlgX in alginate acetylation. J Biol Chem. 2013 Aug 02; 288(31):22299-314. PMID: 23779107; PMCID: PMC3829321.
    41. Koo J, Tang T, Harvey H, Tammam S, Sampaleanu L, Burrows LL, Howell PL. Functional mapping of PilF and PilQ in the Pseudomonas aeruginosa type IV pilus system. Biochemistry. 2013 Apr 30; 52(17):2914-23. PMID: 23547883.
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    42. Ali SS, Whitney JC, Stevenson J, Robinson H, Howell PL, Navarre WW. Structural insights into the regulation of foreign genes in Salmonella by the Hha/H-NS complex. J Biol Chem. 2013 May 10; 288(19):13356-69. PMID: 23515315.
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    43. Colvin KM, Alnabelseya N, Baker P, Whitney JC, Howell PL, Parsek MR. PelA deacetylase activity is required for Pel polysaccharide synthesis in Pseudomonas aeruginosa. J Bacteriol. 2013 May; 195(10):2329-39. PMID: 23504011; PMCID: PMC3650530.
    44. Tammam S, Sampaleanu LM, Koo J, Manoharan K, Daubaras M, Burrows LL, Howell PL. PilMNOPQ from the Pseudomonas aeruginosa type IV pilus system form a transenvelope protein interaction network that interacts with PilA. J Bacteriol. 2013 May; 195(10):2126-35. PMID: 23457250; PMCID: PMC3650547.
    45. Takhar HK, Kemp K, Kim M, Howell PL, Burrows LL. The platform protein is essential for type IV pilus biogenesis. J Biol Chem. 2013 Apr 05; 288(14):9721-8. PMID: 23413032; PMCID: PMC3617274.
    46. Chibba A, Poloczek J, Little DJ, Howell PL, Nitz M. Synthesis and evaluation of inhibitors of E. coli PgaB, a polysaccharide de-N-acetylase involved in biofilm formation. Org Biomol Chem. 2012 Sep 21; 10(35):7103-7. PMID: 22855025.
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    47. Little DJ, Poloczek J, Whitney JC, Robinson H, Nitz M, Howell PL. The structure- and metal-dependent activity of Escherichia coli PgaB provides insight into the partial de-N-acetylation of poly-ß-1,6-N-acetyl-D-glucosamine. J Biol Chem. 2012 Sep 07; 287(37):31126-37. PMID: 22810235.
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    48. Little DJ, Whitney JC, Robinson H, Yip P, Nitz M, Howell PL. Combining in situ proteolysis and mass spectrometry to crystallize Escherichia coli PgaB. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 Jul 01; 68(Pt 7):842-5. PMID: 22750880.
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    49. Whitney JC, Colvin KM, Marmont LS, Robinson H, Parsek MR, Howell PL. Structure of the cytoplasmic region of PelD, a degenerate diguanylate cyclase receptor that regulates exopolysaccharide production in Pseudomonas aeruginosa. J Biol Chem. 2012 Jul 06; 287(28):23582-93. PMID: 22605337; PMCID: PMC3390633.
    50. Wolfram F, Arora K, Robinson H, Neculai AM, Yip P, Howell PL. Expression, purification, crystallization and preliminary X-ray analysis of Pseudomonas aeruginosa AlgL. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 May 01; 68(Pt 5):584-7. PMID: 22691793.
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    51. Marmont LS, Whitney JC, Robinson H, Colvin KM, Parsek MR, Howell PL. Expression, purification, crystallization and preliminary X-ray analysis of Pseudomonas aeruginosa PelD. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 Feb 01; 68(Pt 2):181-4. PMID: 22297994.
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    52. Colvin KM, Irie Y, Tart CS, Urbano R, Whitney JC, Ryder C, Howell PL, Wozniak DJ, Parsek MR. The Pel and Psl polysaccharides provide Pseudomonas aeruginosa structural redundancy within the biofilm matrix. Environ Microbiol. 2012 Aug; 14(8):1913-28. PMID: 22176658; PMCID: PMC3840794.
    53. Broom A, Doxey AC, Lobsanov YD, Berthin LG, Rose DR, Howell PL, McConkey BJ, Meiering EM. Modular evolution and the origins of symmetry: reconstruction of a three-fold symmetric globular protein. Structure. 2012 Jan 11; 20(1):161-71. PMID: 22178248.
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    54. Koo J, Burrows LL, Howell PL. Decoding the roles of pilotins and accessory proteins in secretin escort services. FEMS Microbiol Lett. 2012 Mar; 328(1):1-12. PMID: 22098485.
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    55. Franklin MJ, Nivens DE, Weadge JT, Howell PL. Biosynthesis of the Pseudomonas aeruginosa Extracellular Polysaccharides, Alginate, Pel, and Psl. Front Microbiol. 2011; 2:167. PMID: 21991261; PMCID: PMC3159412.
    56. Whitney JC, Hay ID, Li C, Eckford PD, Robinson H, Amaya MF, Wood LF, Ohman DE, Bear CE, Rehm BH, Howell PL. Structural basis for alginate secretion across the bacterial outer membrane. Proc Natl Acad Sci U S A. 2011 Aug 09; 108(32):13083-8. PMID: 21778407.
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    57. Wehbi H, Portillo E, Harvey H, Shimkoff AE, Scheurwater EM, Howell PL, Burrows LL. The peptidoglycan-binding protein FimV promotes assembly of the Pseudomonas aeruginosa type IV pilus secretin. J Bacteriol. 2011 Jan; 193(2):540-50. PMID: 21097635; PMCID: PMC3019827.
    58. Farrar CE, Siu KK, Howell PL, Jarrett JT. Biotin synthase exhibits burst kinetics and multiple turnovers in the absence of inhibition by products and product-related biomolecules. Biochemistry. 2010 Nov 23; 49(46):9985-96. PMID: 20961145.
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    59. Pell LG, Gasmi-Seabrook GM, Morais M, Neudecker P, Kanelis V, Bona D, Donaldson LW, Edwards AM, Howell PL, Davidson AR, Maxwell KL. The solution structure of the C-terminal Ig-like domain of the bacteriophage ? tail tube protein. J Mol Biol. 2010 Oct 29; 403(3):468-79. PMID: 20826161.
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    60. Ayers M, Howell PL, Burrows LL. Architecture of the type II secretion and type IV pilus machineries. Future Microbiol. 2010 Aug; 5(8):1203-18. PMID: 20722599.
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    61. Siu KK, Asmus K, Zhang AN, Horvatin C, Li S, Liu T, Moffatt B, Woods VL, Howell PL. Mechanism of substrate specificity in 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidases. J Struct Biol. 2011 Jan; 173(1):86-98. PMID: 20554051; PMCID: PMC3006453.
    62. Weadge JT, Yip PP, Robinson H, Arnett K, Tipton PA, Howell PL. Expression, purification, crystallization and preliminary X-ray analysis of Pseudomonas aeruginosa AlgX. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 May 01; 66(Pt 5):588-91. PMID: 20445266.
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    63. Keiski CL, Harwich M, Jain S, Neculai AM, Yip P, Robinson H, Whitney JC, Riley L, Burrows LL, Ohman DE, Howell PL. AlgK is a TPR-containing protein and the periplasmic component of a novel exopolysaccharide secretin. Structure. 2010 Feb 10; 18(2):265-73. PMID: 20159471.
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    64. Pell LG, Liu A, Edmonds L, Donaldson LW, Howell PL, Davidson AR. The X-ray crystal structure of the phage lambda tail terminator protein reveals the biologically relevant hexameric ring structure and demonstrates a conserved mechanism of tail termination among diverse long-tailed phages. J Mol Biol. 2009 Jun 26; 389(5):938-51. PMID: 19426744.
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    65. Whitney JC, Neculai AM, Ohman DE, Howell PL. Expression, refolding, crystallization and preliminary X-ray analysis of Pseudomonas aeruginosa AlgE. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2009 May 01; 65(Pt 5):463-6. PMID: 19407377; PMCID: PMC2675585.
    66. Pell LG, Kanelis V, Donaldson LW, Howell PL, Davidson AR. The phage lambda major tail protein structure reveals a common evolution for long-tailed phages and the type VI bacterial secretion system. Proc Natl Acad Sci U S A. 2009 Mar 17; 106(11):4160-5. PMID: 19251647; PMCID: PMC2657425.
    67. Rodinger T, Howell PL, Pomès R. Calculation of absolute protein-ligand binding free energy using distributed replica sampling. J Chem Phys. 2008 Oct 21; 129(15):155102. PMID: 19045232.
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    68. Koo J, Tammam S, Ku SY, Sampaleanu LM, Burrows LL, Howell PL. PilF is an outer membrane lipoprotein required for multimerization and localization of the Pseudomonas aeruginosa Type IV pilus secretin. J Bacteriol. 2008 Nov; 190(21):6961-9. PMID: 18776008; PMCID: PMC2580691.
    69. Zarrine-Afsar A, Wallin S, Neculai AM, Neudecker P, Howell PL, Davidson AR, Chan HS. Theoretical and experimental demonstration of the importance of specific nonnative interactions in protein folding. Proc Natl Acad Sci U S A. 2008 Jul 22; 105(29):9999-10004. PMID: 18626019; PMCID: PMC2481363.
    70. Siu KK, Lee JE, Smith GD, Horvatin-Mrakovcic C, Howell PL. Structure of Staphylococcus aureus 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 May 01; 64(Pt 5):343-50. PMID: 18453700; PMCID: PMC2376413.
    71. Lobsanov YD, Yoshida T, Desmet T, Nerinckx W, Yip P, Claeyssens M, Herscovics A, Howell PL. Modulation of activity by Arg407: structure of a fungal alpha-1,2-mannosidase in complex with a substrate analogue. Acta Crystallogr D Biol Crystallogr. 2008 Mar; 64(Pt 3):227-36. PMID: 18323617.
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    72. Siu KK, Lee JE, Sufrin JR, Moffatt BA, McMillan M, Cornell KA, Isom C, Howell PL. Molecular determinants of substrate specificity in plant 5'-methylthioadenosine nucleosidases. J Mol Biol. 2008 Apr 18; 378(1):112-28. PMID: 18342331; PMCID: PMC2908701.
    73. Chiang P, Sampaleanu LM, Ayers M, Pahuta M, Howell PL, Burrows LL. Functional role of conserved residues in the characteristic secretion NTPase motifs of the Pseudomonas aeruginosa type IV pilus motor proteins PilB, PilT and PilU. Microbiology. 2008 Jan; 154(Pt 1):114-26. PMID: 18174131.
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    74. Ku SY, Cornell KA, Howell PL. Structure of Arabidopsis thaliana 5-methylthioribose kinase reveals a more occluded active site than its bacterial homolog. BMC Struct Biol. 2007 Oct 25; 7:70. PMID: 17961230; PMCID: PMC2194712.
    75. Ku SY, Yip P, Cornell KA, Riscoe MK, Behr JB, Guillerm G, Howell PL. Structures of 5-methylthioribose kinase reveal substrate specificity and unusual mode of nucleotide binding. J Biol Chem. 2007 Jul 27; 282(30):22195-206. PMID: 17522047.
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    76. Tsai M, Koo J, Yip P, Colman RF, Segall ML, Howell PL. Substrate and product complexes of Escherichia coli adenylosuccinate lyase provide new insights into the enzymatic mechanism. J Mol Biol. 2007 Jul 13; 370(3):541-54. PMID: 17531264; PMCID: PMC4113493.
    77. Keiski CL, Yip P, Robinson H, Burrows LL, Howell PL. Expression, purification, crystallization and preliminary X-ray analysis of Pseudomonas fluorescens AlgK. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 May 01; 63(Pt 5):415-8. PMID: 17565185; PMCID: PMC2335008.
    78. Ku SY, Smith GD, Howell PL. ADP-2Ho as a phasing tool for nucleotide-containing proteins. Acta Crystallogr D Biol Crystallogr. 2007 Apr; 63(Pt 4):493-9. PMID: 17372354.
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    79. Smith GD, Lemke CT, Howell PL. Substructure determination in multiwavelength anomalous diffraction, single anomalous diffraction, and single isomorphous replacement with anomalous scattering data using Shake-and-Bake. Methods Mol Biol. 2007; 364:183-96. PMID: 17172766.
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    80. Ku SY, Yip P, Howell PL. Structure of Escherichia coli tryptophanase. Acta Crystallogr D Biol Crystallogr. 2006 Jul; 62(Pt 7):814-23. PMID: 16790938.
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    81. Rodinger T, Howell PL, Pomès R. Distributed Replica Sampling. J Chem Theory Comput. 2006 May; 2(3):725-31. PMID: 26626677.
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    82. Lee JE, Smith GD, Horvatin C, Huang DJ, Cornell KA, Riscoe MK, Howell PL. Structural snapshots of MTA/AdoHcy nucleosidase along the reaction coordinate provide insights into enzyme and nucleoside flexibility during catalysis. J Mol Biol. 2005 Sep 23; 352(3):559-74. PMID: 16109423.
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    83. Singh V, Lee JE, Núñez S, Howell PL, Schramm VL. Transition state structure of 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase from Escherichia coli and its similarity to transition state analogues. Biochemistry. 2005 Sep 06; 44(35):11647-59. PMID: 16128565.
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    84. Lee JE, Luong W, Huang DJ, Cornell KA, Riscoe MK, Howell PL. Mutational analysis of a nucleosidase involved in quorum-sensing autoinducer-2 biosynthesis. Biochemistry. 2005 Aug 23; 44(33):11049-57. PMID: 16101288.
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    85. Rodinger T, Howell PL, Pomès R. Absolute free energy calculations by thermodynamic integration in four spatial dimensions. J Chem Phys. 2005 Jul 15; 123(3):34104. PMID: 16080727.
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    86. Tsai M, Koo J, Howell PL. Recovery of argininosuccinate lyase activity in duck delta1 crystallin. Biochemistry. 2005 Jun 28; 44(25):9034-44. PMID: 15966727.
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    87. Singh V, Evans GB, Lenz DH, Mason JM, Clinch K, Mee S, Painter GF, Tyler PC, Furneaux RH, Lee JE, Howell PL, Schramm VL. Femtomolar transition state analogue inhibitors of 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase from Escherichia coli. J Biol Chem. 2005 May 06; 280(18):18265-73. PMID: 15749708.
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    88. Lee JE, Singh V, Evans GB, Tyler PC, Furneaux RH, Cornell KA, Riscoe MK, Schramm VL, Howell PL. Structural rationale for the affinity of pico- and femtomolar transition state analogues of Escherichia coli 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase. J Biol Chem. 2005 May 06; 280(18):18274-82. PMID: 15746096.
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    89. Sampaleanu LM, Codding PW, Lobsanov YD, Tsai M, Smith GD, Horvatin C, Howell PL. Structural studies of duck delta2 crystallin mutants provide insight into the role of Thr161 and the 280s loop in catalysis. Biochem J. 2004 Dec 01; 384(Pt 2):437-47. PMID: 15320872.
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    90. Tsai M, Sampaleanu LM, Greene C, Creagh L, Haynes C, Howell PL. A duck delta1 crystallin double loop mutant provides insight into residues important for argininosuccinate lyase activity. Biochemistry. 2004 Sep 21; 43(37):11672-82. PMID: 15362851.
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    91. Yu B, Paroutis P, Davidson AR, Howell PL. Disruption of a salt bridge dramatically accelerates subunit exchange in duck delta2 crystallin. J Biol Chem. 2004 Sep 24; 279(39):40972-9. PMID: 15273245.
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    92. Lee JE, Settembre EC, Cornell KA, Riscoe MK, Sufrin JR, Ealick SE, Howell PL. Structural comparison of MTA phosphorylase and MTA/AdoHcy nucleosidase explains substrate preferences and identifies regions exploitable for inhibitor design. Biochemistry. 2004 May 11; 43(18):5159-69. PMID: 15122881.
      View in: PubMed
    93. Lobsanov YD, Romero PA, Sleno B, Yu B, Yip P, Herscovics A, Howell PL. Structure of Kre2p/Mnt1p: a yeast alpha1,2-mannosyltransferase involved in mannoprotein biosynthesis. J Biol Chem. 2004 Apr 23; 279(17):17921-31. PMID: 14752117.
      View in: PubMed
    94. Ku SY, Yip P, Cornell KA, Riscoe MK, Howell PL. Crystallization and preliminary X-ray analysis of 5'-methylthioribose kinase from Bacillus subtilis and Arabidopsis thaliana. Acta Crystallogr D Biol Crystallogr. 2004 Jan; 60(Pt 1):116-9. PMID: 14684902.
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    95. Yang X, Hu Y, Yin DH, Turner MA, Wang M, Borchardt RT, Howell PL, Kuczera K, Schowen RL. Catalytic strategy of S-adenosyl-L-homocysteine hydrolase: transition-state stabilization and the avoidance of abortive reactions. Biochemistry. 2003 Feb 25; 42(7):1900-9. PMID: 12590576.
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    96. Lee JE, Cornell KA, Riscoe MK, Howell PL. Structure of Escherichia coli 5'-methylthioadenosine/ S-adenosylhomocysteine nucleosidase inhibitor complexes provide insight into the conformational changes required for substrate binding and catalysis. J Biol Chem. 2003 Mar 07; 278(10):8761-70. PMID: 12496243.
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    97. Lemke CT, Smith GD, Howell PL. S-SAD, Se-SAD and S/Se-SIRAS using Cu Kalpha radiation: why wait for synchrotron time? Acta Crystallogr D Biol Crystallogr. 2002 Dec; 58(Pt 12):2096-101. PMID: 12454470.
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    98. Lemke CT, Howell PL. Substrate induced conformational changes in argininosuccinate synthetase. J Biol Chem. 2002 Apr 12; 277(15):13074-81. PMID: 11809762.
      View in: PubMed
    99. Lobsanov YD, Vallée F, Imberty A, Yoshida T, Yip P, Herscovics A, Howell PL. Structure of Penicillium citrinum alpha 1,2-mannosidase reveals the basis for differences in specificity of the endoplasmic reticulum and Golgi class I enzymes. J Biol Chem. 2002 Feb 15; 277(7):5620-30. PMID: 11714724.
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    100. Sampaleanu LM, Yu B, Howell PL. Mutational analysis of duck delta 2 crystallin and the structure of an inactive mutant with bound substrate provide insight into the enzymatic mechanism of argininosuccinate lyase. J Biol Chem. 2002 Feb 08; 277(6):4166-75. PMID: 11698398.
      View in: PubMed
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