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Roman Melnyk

TitleSenior Scientist
InstitutionThe Hospital for Sick Children
DepartmentMolecular Medicine
Address555 University Avenue
Toronto ON M5G 1X8
Phone416-813-1500
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    Collapse Bibliographic 
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    Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Faculty can login to make corrections and additions.
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    1. Jardine S, Anderson S, Babcock S, Leung G, Pan J, Dhingani N, Warner N, Guo C, Siddiqui I, Kotlarz D, Dowling JJ, Melnyk R, Snapper SB, Klein C, Thiagarajah JR, Muise AM. Drug Screen Identifies Leflunomide for Treatment of Inflammatory Bowel Diseases Caused by TTC7A Deficiency. Gastroenterology. 2019 Nov 16. PMID: 31743734.
      View in: PubMed
    2. Icho S, Melnyk RA. Dismantling a Toxin to Disarm a Superbug. Trends Pharmacol Sci. 2019 03; 40(3):155-156. PMID: 30685063.
      View in: PubMed
    3. Tam J, Hamza T, Ma B, Chen K, Beilhartz GL, Ravel J, Feng H, Melnyk RA. Host-targeted niclosamide inhibits C. difficile virulence and prevents disease in mice without disrupting the gut microbiota. Nat Commun. 2018 12 07; 9(1):5233. PMID: 30531960.
      View in: PubMed
    4. Park M, Xu X, Min W, Sugiman-Marangos SN, Beilhartz GL, Adams JJ, Sidhu SS, Grunebaum E, Melnyk RA. Intracellular Delivery of Human Purine Nucleoside Phosphorylase by Engineered Diphtheria Toxin Rescues Function in Target Cells. Mol Pharm. 2018 11 05; 15(11):5217-5226. PMID: 30212635.
      View in: PubMed
    5. Mansfield MJ, Sugiman-Marangos SN, Melnyk RA, Doxey AC. Identification of a diphtheria toxin-like gene family beyond the Corynebacterium genus. FEBS Lett. 2018 08; 592(16):2693-2705. PMID: 30058084.
      View in: PubMed
    6. Orrell KE, Tellgren-Roth Å, Di Bernardo M, Zhang Z, Cuviello F, Lundqvist J, von Heijne G, Nilsson I, Melnyk RA. Direct Detection of Membrane-Inserting Fragments Defines the Translocation Pores of a Family of Pathogenic Toxins. J Mol Biol. 2018 09 14; 430(18 Pt B):3190-3199. PMID: 29990469.
      View in: PubMed
    7. Lai MT, Tawa P, Auger A, Wang D, Su HP, Yan Y, Hazuda DJ, Miller MD, Asante-Appiah E, Melnyk RA. Identification of novel bifunctional HIV-1 reverse transcriptase inhibitors. J Antimicrob Chemother. 2018 Jan 01; 73(1):109-117. PMID: 29029095.
      View in: PubMed
    8. Kroh HK, Chandrasekaran R, Zhang Z, Rosenthal K, Woods R, Jin X, Nyborg AC, Rainey GJ, Warrener P, Melnyk RA, Spiller BW, Lacy DB. A neutralizing antibody that blocks delivery of the enzymatic cargo of Clostridium difficile toxin TcdB into host cells. J Biol Chem. 2018 01 19; 293(3):941-952. PMID: 29180448.
      View in: PubMed
    9. Gupta P, Zhang Z, Sugiman-Marangos SN, Tam J, Raman S, Julien JP, Kroh HK, Lacy DB, Murgolo N, Bekkari K, Therien AG, Hernandez LD, Melnyk RA. Functional defects in Clostridium difficile TcdB toxin uptake identify CSPG4 receptor-binding determinants. J Biol Chem. 2017 10 20; 292(42):17290-17301. PMID: 28842504.
      View in: PubMed
    10. Orrell KE, Zhang Z, Sugiman-Marangos SN, Melnyk RA. Clostridium difficile toxins A and B: Receptors, pores, and translocation into cells. Crit Rev Biochem Mol Biol. 2017 08; 52(4):461-473. PMID: 28545305.
      View in: PubMed
    11. Beilhartz GL, Sugiman-Marangos SN, Melnyk RA. Repurposing bacterial toxins for intracellular delivery of therapeutic proteins. Biochem Pharmacol. 2017 10 15; 142:13-20. PMID: 28408344.
      View in: PubMed
    12. Beilhartz GL, Tam J, Zhang Z, Melnyk RA. Comment on "A small-molecule antivirulence agent for treating Clostridium difficile infection". Sci Transl Med. 2016 12 21; 8(370):370tc2. PMID: 28003550.
      View in: PubMed
    13. Baker P, Hill PJ, Snarr BD, Alnabelseya N, Pestrak MJ, Lee MJ, Jennings LK, Tam J, Melnyk RA, Parsek MR, Sheppard DC, Wozniak DJ, Howell PL. Exopolysaccharide biosynthetic glycoside hydrolases can be utilized to disrupt and prevent Pseudomonas aeruginosa biofilms. Sci Adv. 2016 05; 2(5):e1501632. PMID: 27386527.
      View in: PubMed
    14. Chumbler NM, Rutherford SA, Zhang Z, Farrow MA, Lisher JP, Farquhar E, Giedroc DP, Spiller BW, Melnyk RA, Lacy DB. Crystal structure of Clostridium difficile toxin A. Nat Microbiol. 2016; 1. PMID: 27512603.
      View in: PubMed
    15. Chumbler NM, Rutherford SA, Zhang Z, Farrow MA, Lisher JP, Farquhar E, Giedroc DP, Spiller BW, Melnyk RA, Lacy DB. Crystal structure of Clostridium difficile toxin A. Nat Microbiol. 2016 Jan 11; 1:15002. PMID: 27571750.
      View in: PubMed
    16. Beilhartz GL, Tam J, Melnyk RA. Small Molecules Take A Big Step Against Clostridium difficile. Trends Microbiol. 2015 Dec; 23(12):746-748. PMID: 26547239.
      View in: PubMed
    17. Hamza T, Zhang Z, Melnyk RA, Feng H. Defective mutations within the translocation domain of Clostridium difficile toxin B impair disease pathogenesis. Pathog Dis. 2016 Feb; 74(1):ftv098. PMID: 26507679.
      View in: PubMed
    18. Auger A, Park M, Nitschke F, Minassian LM, Beilhartz GL, Minassian BA, Melnyk RA. Efficient Delivery of Structurally Diverse Protein Cargo into Mammalian Cells by a Bacterial Toxin. Mol Pharm. 2015 Aug 03; 12(8):2962-71. PMID: 26103531.
      View in: PubMed
    19. Tam J, Beilhartz GL, Auger A, Gupta P, Therien AG, Melnyk RA. Small molecule inhibitors of Clostridium difficile toxin B-induced cellular damage. Chem Biol. 2015 Feb 19; 22(2):175-85. PMID: 25619932.
      View in: PubMed
    20. Bernatchez JA, Paul R, Tchesnokov EP, Ngure M, Beilhartz GL, Berghuis AM, Lavoie R, Li L, Auger A, Melnyk RA, Grobler JA, Miller MD, Hazuda DJ, Hecht SM, Götte M. Derivatives of mesoxalic acid block translocation of HIV-1 reverse transcriptase. J Biol Chem. 2015 Jan 16; 290(3):1474-84. PMID: 25355312.
      View in: PubMed
    21. Zhang Z, Park M, Tam J, Auger A, Beilhartz GL, Lacy DB, Melnyk RA. Translocation domain mutations affecting cellular toxicity identify the Clostridium difficile toxin B pore. Proc Natl Acad Sci U S A. 2014 Mar 11; 111(10):3721-6. PMID: 24567384.
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    22. Lam V, Henault M, Khougaz K, Fortin LJ, Ouellet M, Melnyk R, Partridge A. Resorufin butyrate as a soluble and monomeric high-throughput substrate for a triglyceride lipase. J Biomol Screen. 2012 Feb; 17(2):245-51. PMID: 21956174.
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    23. Auger A, Beilhartz GL, Zhu S, Cauchon E, Falgueyret JP, Grobler JA, Ehteshami M, Götte M, Melnyk RA. Impact of primer-induced conformational dynamics of HIV-1 reverse transcriptase on polymerase translocation and inhibition. J Biol Chem. 2011 Aug 26; 286(34):29575-83. PMID: 21737446.
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    24. Cauchon E, Falgueyret JP, Auger A, Melnyk RA. A high-throughput continuous assay for screening and characterization of inhibitors of HIV reverse-transcriptase DNA polymerase activity. J Biomol Screen. 2011 Jun; 16(5):518-24. PMID: 21474837.
      View in: PubMed
    25. Tam J, Henault M, Li L, Wang Z, Partridge AW, Melnyk RA. An activity-based probe for high-throughput measurements of triacylglycerol lipases. Anal Biochem. 2011 Jul 15; 414(2):254-60. PMID: 21397586.
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    26. Melnyk RA, Tam J, Boie Y, Kennedy BP, Percival MD. Renin and prorenin activate pathways implicated in organ damage in human mesangial cells independent of angiotensin II production. Am J Nephrol. 2009; 30(3):232-43. PMID: 19451703.
      View in: PubMed
    27. Gorman PM, Kim S, Guo M, Melnyk RA, McLaurin J, Fraser PE, Bowie JU, Chakrabartty A. Dimerization of the transmembrane domain of amyloid precursor proteins and familial Alzheimer's disease mutants. BMC Neurosci. 2008 Jan 30; 9:17. PMID: 18234110.
      View in: PubMed
    28. Wimalasena DS, Cramer JC, Janowiak BE, Juris SJ, Melnyk RA, Anderson DE, Kirk KL, Collier RJ, Bann JG. Effect of 2-fluorohistidine labeling of the anthrax protective antigen on stability, pore formation, and translocation. Biochemistry. 2007 Dec 25; 46(51):14928-36. PMID: 18044973.
      View in: PubMed
    29. Juris SJ, Melnyk RA, Bolcome RE, Chan J, Collier RJ. Cross-linked forms of the isolated N-terminal domain of the lethal factor are potent inhibitors of anthrax toxin. Infect Immun. 2007 Oct; 75(10):5052-8. PMID: 17635861.
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    30. Johnson RM, Rath A, Melnyk RA, Deber CM. Lipid solvation effects contribute to the affinity of Gly-xxx-Gly motif-mediated helix-helix interactions. Biochemistry. 2006 Jul 18; 45(28):8507-15. PMID: 16834324.
      View in: PubMed
    31. Melnyk RA, Collier RJ. A loop network within the anthrax toxin pore positions the phenylalanine clamp in an active conformation. Proc Natl Acad Sci U S A. 2006 Jun 27; 103(26):9802-7. PMID: 16785422.
      View in: PubMed
    32. Go MY, Kim S, Partridge AW, Melnyk RA, Rath A, Deber CM, Mogridge J. Self-association of the transmembrane domain of an anthrax toxin receptor. J Mol Biol. 2006 Jun 30; 360(1):145-56. PMID: 16756998.
      View in: PubMed
    33. Rath A, Melnyk RA, Deber CM. Evidence for assembly of small multidrug resistance proteins by a "two-faced" transmembrane helix. J Biol Chem. 2006 Jun 02; 281(22):15546-53. PMID: 16574656.
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    34. Melnyk RA, Hewitt KM, Lacy DB, Lin HC, Gessner CR, Li S, Woods VL, Collier RJ. Structural determinants for the binding of anthrax lethal factor to oligomeric protective antigen. J Biol Chem. 2006 Jan 20; 281(3):1630-5. PMID: 16293620.
      View in: PubMed
    35. Lacy DB, Lin HC, Melnyk RA, Schueler-Furman O, Reither L, Cunningham K, Baker D, Collier RJ. A model of anthrax toxin lethal factor bound to protective antigen. Proc Natl Acad Sci U S A. 2005 Nov 08; 102(45):16409-14. PMID: 16251269.
      View in: PubMed
    36. Krantz BA, Melnyk RA, Zhang S, Juris SJ, Lacy DB, Wu Z, Finkelstein A, Collier RJ. A phenylalanine clamp catalyzes protein translocation through the anthrax toxin pore. Science. 2005 Jul 29; 309(5735):777-81. PMID: 16051798.
      View in: PubMed
    37. Christensen KA, Krantz BA, Melnyk RA, Collier RJ. Interaction of the 20 kDa and 63 kDa fragments of anthrax protective antigen: kinetics and thermodynamics. Biochemistry. 2005 Jan 25; 44(3):1047-53. PMID: 15654761.
      View in: PubMed
    38. Lacy DB, Wigelsworth DJ, Melnyk RA, Harrison SC, Collier RJ. Structure of heptameric protective antigen bound to an anthrax toxin receptor: a role for receptor in pH-dependent pore formation. Proc Natl Acad Sci U S A. 2004 Sep 07; 101(36):13147-51. PMID: 15326297.
      View in: PubMed
    39. Melnyk RA, Kim S, Curran AR, Engelman DM, Bowie JU, Deber CM. The affinity of GXXXG motifs in transmembrane helix-helix interactions is modulated by long-range communication. J Biol Chem. 2004 Apr 16; 279(16):16591-7. PMID: 14766751.
      View in: PubMed
    40. Dawson JP, Melnyk RA, Deber CM, Engelman DM. Sequence context strongly modulates association of polar residues in transmembrane helices. J Mol Biol. 2003 Aug 01; 331(1):255-62. PMID: 12875850.
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    41. Partridge AW, Melnyk RA, Yang D, Bowie JU, Deber CM. A transmembrane segment mimic derived from Escherichia coli diacylglycerol kinase inhibits protein activity. J Biol Chem. 2003 Jun 13; 278(24):22056-60. PMID: 12686537.
      View in: PubMed
    42. Maeda M, Melnyk RA, Partridge AW, Liu LP, Deber CM. Transmembrane segment peptides with double D-amino acid replacements: helicity, hydrophobicity, and antimicrobial activity. Biopolymers. 2003; 71(1):77-84. PMID: 12712502.
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    43. Melnyk RA, Partridge AW, Yip J, Wu Y, Goto NK, Deber CM. Polar residue tagging of transmembrane peptides. Biopolymers. 2003; 71(6):675-85. PMID: 14991677.
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    44. Partridge AW, Melnyk RA, Deber CM. Polar residues in membrane domains of proteins: molecular basis for helix-helix association in a mutant CFTR transmembrane segment. Biochemistry. 2002 Mar 19; 41(11):3647-53. PMID: 11888281.
      View in: PubMed
    45. Melnyk RA, Partridge AW, Deber CM. Transmembrane domain mediated self-assembly of major coat protein subunits from Ff bacteriophage. J Mol Biol. 2002 Jan 04; 315(1):63-72. PMID: 11771966.
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    46. Melnyk RA, Partridge AW, Deber CM. Retention of native-like oligomerization states in transmembrane segment peptides: application to the Escherichia coli aspartate receptor. Biochemistry. 2001 Sep 18; 40(37):11106-13. PMID: 11551208.
      View in: PubMed
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