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Jane McGlade

TitleSenior Scientist
InstitutionThe Hospital for Sick Children
DepartmentCell Biology
Address555 University Avenue
Toronto ON M5G 1X8
Phone416-813-1500
vCardDownload vCard

    Collapse Biography 
    Collapse education and training
    University of Guelph, Guelph ONB.Sc1983Biochemistry
    McMaster University, Hamilton ONPh.D1990Molecular Virology and Immunology
    Lunenfeld Research Institute, University of Toronto, Toronto ONPost-Doc Fellow1994Tyrosine Kinase Signaling
    Collapse awards and honors
    1986 - 1990National Cancer Institute Studentship
    1990 - 1994National Cancer Institute Fellowship
    1999Premiere’s Research Excellence Award
    1999Harold E. Johns Award, NCIC
    1999 - 2005Canadian Cancer Society Research Scientist , Canadian Cancer Research Society
    2001Award for Leukemia Research, Leukemia Research Fund of Canada
    2004McLaughlin Scholar, R. Samuel McLaughlin Centre for Molecular Medicine
    2004Award for Leukemia Research, Leukemia Research Fund of Canada
    2008Award for Leukemia Research, Leukemia and Lymphoma Society of Canada

    Collapse Overview 
    Collapse overview
    My research is directed towards understanding the regulation of protein interactions and signal-transduction pathways that control normal cellular functions, and that are disrupted in human disease. Projects in the lab are currently focused on three main themes: phospho-proteomic profiling of tyrosine kinase driven leukaemia; ubiquitin dependent regulation of oncogenic signaling and mechanisms of E3 ligase activity; regulation of alternative splicing by oncogenic signalling and specificity of protein isofom function.

    Regulation signal transduction by modular adaptor proteins.
    My colleagues and I have focused the identification and functional characterization of intracellular adapter proteins that function to integrate, localize, and down regulate signal-transduction cascades. My laboratory has cloned and characterized novel adapters that function in antigen-receptor and cytokine signaling (GADS, SLAP), and others that regulate the activity of trans-membrane receptors that determine cell fate and polarity during development (NUMB). Work in my laboratory focuses on detailed molecular and functional characterization of these molecules, including their post translational modifications, isoform specific functions, their associated protein networks and functions in brain and blood cancers. Currently, we are also investigating the developmental signaling and oncogenic pathways that regulate the expression of Numb adaptor isoforms through alternative splicing.

    Ubiquitin dependent regulation of cell polarity and vesicle trafficking.
    Our lab is studying the role of ubiquitin in the regulation of signaling pathways and trafficking of surface receptors. We discovered a family of novel PDZ domain containing E3 ubiquitin ligases that includes LNX and LNX2and determined the function of LNX and LNX2 activity in cell fate determination and the establishment of cell polarity during embryonic development. A second small family of E3 ligases currently under investigation by our group includes RNF126 and RabRING7. We have found that these ligases can mediate the formation of both K48 and K63 linked chains and regulate late endosome sorting of receptor tyrosine kinases. Most recently we have discovered a novel mechanism that regulates the ubiquitin ligase activity of CBL and developed in vitro approaches to identify potential therapeutics for diseases that are driven by activated tyrosine kinases.

    Development of high-throughput functional protein assays and molecular tool archives.
    In collaboration with Daniela Rotin in the Department of Biochemistry, I developed a Canadian Foundation for Innovation funded functional proteomics program. In the first phase, we assembled the SIDNET Protein Archive of ~14,000 human proteins and developed high throughput assays to measure protein function using protein arrays and high throughput detection and imaging systems. The SIDNET platform capabilities were then expanded to include mouse and human shRNA and esiRNA libraries, and most recently, small molecule and drug libraries. Currently my group is establishing a high throughput assay to identify oncogenic and developmental signaling pathways that regulate alternative splicing events as well as small molecule regulators of ubiquitin ligases. In 2013, SIDNET merged with the Advanced Protein Technology Centre and became the SPARC Biocentre.
    (http://www.sickkids.ca/Research/SPARC/about-us/index.html)


    Collapse Research 
    Collapse research activities and funding
         (Jane McGlade)Apr 1, 2014 - Mar 31, 2019
    NSERC
    Ubiquitin System regulation of epithelial polarity
    Role: Principal applicant

         (Jane McGlade)Oct 1, 2015 - Mar 31, 2019
    b.r.a.i.n.child
    Alternative splicing of the Numb tumor suppressor in medulloblastoma
    Role: Principal applicant

         (Jane McGlade)Oct 1, 2015 - Jun 3, 2020
    Canadian Institutes of Health Research (CIHR)
    Function and regulation of Numb isoform expression in cancer
    Role: Principal applicant

         (Elise Heon)Apr 1, 2016 - Mar 31, 2019
    Foundation Fighting Blindness Canada
    Developing a strategic multidisciplinary approach to target the genetic basis of photoreceptor degeneration: a proof of principle
    Role: Co-Investigator

         (Jane McGlade)Apr 1, 2017 - Mar 31, 2019
    Canadian Institutes of Health Research (CIHR)
    Identifying novel signaling pathways in BCR-ABL driven leukemia

         (Daniela Rotin)Apr 1, 2017 - Mar 31, 2022
    Canadian Foundation of Innovation (CFI)
    3D-ORG: 3D screening infrastructure for tissue ORGanoids and model ORGanisms
    Role: Co-Investigators

         (Michael Moran)Apr 1, 2017 - May 31, 2022
    Canadian Foundation of Innovation (CFI)
    Infrastructure for the analysis of disease proteins
    Role: Co-Investigators

         (Jane McGlade)Sep 1, 2017 - Aug 31, 2019
    Cancer Research Society
    Approaches to regulate Cbl ubiquitin ligase activity in leukemia
    Role: Principal applicant

         (Jane McGlade)Oct 1, 2017 - Sep 30, 2018
    Meagan's Walk
    Approaches to targetting of alternative splicing in pediatric brain tumours
    Role: Principal applicant

         (Jane McGlade)Jun 1, 2018 - May 31, 2020
    Megan's Walk
    Splicing Regulation of Tumour Suppressor Activity in Pediatric Brain Cancer
    Role: Principal applicant

    Collapse Bibliographic 
    Collapse selected publications
    Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Faculty can login to make corrections and additions.
    List All   |   Timeline
    1. Sapir T, Levy T, Kozer N, Shin I, Zamor V, Haffner-Krausz R, McGlade JC, Reiner O. Notch Activation by Shootin1 Opposing Activities on 2 Ubiquitin Ligases. Cereb Cortex. 2018 Sep 01; 28(9):3115-3128. PMID: 28981589.
      View in: PubMed
    2. Smith TL, Van Slyke P, Jones N, Dumont DJ, McGlade CJ. Tie2 signalling through Erk1/2 regulates TLR4 driven inflammation. Cell Signal. 2018 Nov; 51:211-221. PMID: 30077653.
      View in: PubMed
    3. Hirai M, Arita Y, McGlade CJ, Lee KF, Chen J, Evans SM. Adaptor proteins NUMB and NUMBL promote cell cycle withdrawal by targeting ERBB2 for degradation. J Clin Invest. 2017 Feb 01; 127(2):569-582. PMID: 28067668.
      View in: PubMed
    4. Torchia J, Golbourn B, Feng S, Ho KC, Sin-Chan P, Vasiljevic A, Norman JD, Guilhamon P, Garzia L, Agamez NR, Lu M, Chan TS, Picard D, de Antonellis P, Khuong-Quang DA, Planello AC, Zeller C, Barsyte-Lovejoy D, Lafay-Cousin L, Letourneau L, Bourgey M, Yu M, Gendoo DMA, Dzamba M, Barszczyk M, Medina T, Riemenschneider AN, Morrissy AS, Ra YS, Ramaswamy V, Remke M, Dunham CP, Yip S, Ng HK, Lu JQ, Mehta V, Albrecht S, Pimentel J, Chan JA, Somers GR, Faria CC, Roque L, Fouladi M, Hoffman LM, Moore AS, Wang Y, Choi SA, Hansford JR, Catchpoole D, Birks DK, Foreman NK, Strother D, Klekner A, Bognár L, Garami M, Hauser P, Hortobágyi T, Wilson B, Hukin J, Carret AS, Van Meter TE, Hwang EI, Gajjar A, Chiou SH, Nakamura H, Toledano H, Fried I, Fults D, Wataya T, Fryer C, Eisenstat DD, Scheinemann K, Fleming AJ, Johnston DL, Michaud J, Zelcer S, Hammond R, Afzal S, Ramsay DA, Sirachainan N, Hongeng S, Larbcharoensub N, Grundy RG, Lulla RR, Fangusaro JR, Druker H, Bartels U, Grant R, Malkin D, McGlade CJ, Nicolaides T, Tihan T, Phillips J, Majewski J, Montpetit A, Bourque G, Bader GD, Reddy AT, Gillespie GY, Warmuth-Metz M, Rutkowski S, Tabori U, Lupien M, Brudno M, Schüller U, Pietsch T, Judkins AR, Hawkins CE, Bouffet E, Kim SK, Dirks PB, Taylor MD, Erdreich-Epstein A, Arrowsmith CH, De Carvalho DD, Rutka JT, Jabado N, Huang A. Integrated (epi)-Genomic Analyses Identify Subgroup-Specific Therapeutic Targets in CNS Rhabdoid Tumors. Cancer Cell. 2016 Dec 12; 30(6):891-908. PMID: 27960086.
      View in: PubMed
    5. Ramkumar N, Omelchenko T, Silva-Gagliardi NF, McGlade CJ, Wijnholds J, Anderson KV. Crumbs2 promotes cell ingression during the epithelial-to-mesenchymal transition at gastrulation. Nat Cell Biol. 2016 Dec; 18(12):1281-1291. PMID: 27870829.
      View in: PubMed
    6. Rajendran D, Zhang Y, Berry DM, McGlade CJ. Regulation of Numb isoform expression by activated ERK signaling. Oncogene. 2016 09 29; 35(39):5202-13. PMID: 27041567.
      View in: PubMed
    7. Chan TS, Hawkins C, Krieger JR, McGlade CJ, Huang A. JPO2/CDCA7L and LEDGF/p75 Are Novel Mediators of PI3K/AKT Signaling and Aggressive Phenotypes in Medulloblastoma. Cancer Res. 2016 05 01; 76(9):2802-12. PMID: 27013196.
      View in: PubMed
    8. Ramkumar N, Harvey BM, Lee JD, Alcorn HL, Silva-Gagliardi NF, McGlade CJ, Bestor TH, Wijnholds J, Haltiwanger RS, Anderson KV. Protein O-Glucosyltransferase 1 (POGLUT1) Promotes Mouse Gastrulation through Modification of the Apical Polarity Protein CRUMBS2. PLoS Genet. 2015 Oct; 11(10):e1005551. PMID: 26496195.
      View in: PubMed
    9. Jin LL, Wybenga-Groot LE, Tong J, Taylor P, Minden MD, Trudel S, McGlade CJ, Moran MF. Tyrosine phosphorylation of the Lyn Src homology 2 (SH2) domain modulates its binding affinity and specificity. Mol Cell Proteomics. 2015 Mar; 14(3):695-706. PMID: 25587033; PMCID: PMC4349988.
    10. Krieger JR, Taylor P, Moran MF, McGlade CJ. Comprehensive identification of phosphorylation sites on the Numb endocytic adaptor protein. Proteomics. 2015 Jan; 15(2-3):434-46. PMID: 25403733.
      View in: PubMed
    11. Wybenga-Groot LE, McGlade CJ. RTK SLAP down: the emerging role of Src-like adaptor protein as a key player in receptor tyrosine kinase signaling. Cell Signal. 2015 Feb; 27(2):267-74. PMID: 25446260.
      View in: PubMed
    12. Wybenga-Groot LE, Ho CS, Sweeney FD, Ceccarelli DF, McGlade CJ, Durocher D, Sicheri F. Structural basis of Rad53 kinase activation by dimerization and activation segment exchange. Cell Signal. 2014 Sep; 26(9):1825-36. PMID: 24815189.
      View in: PubMed
    13. Smith CJ, McGlade CJ. The ubiquitin ligase RNF126 regulates the retrograde sorting of the cation-independent mannose 6-phosphate receptor. Exp Cell Res. 2014 Jan 15; 320(2):219-32. PMID: 24275455.
      View in: PubMed
    14. Wybenga-Groot LE, McGlade CJ. Crystal structure of Src-like adaptor protein 2 reveals close association of SH3 and SH2 domains through ß-sheet formation. Cell Signal. 2013 Dec; 25(12):2702-8. PMID: 24018043.
      View in: PubMed
    15. Smith CJ, Berry DM, McGlade CJ. The E3 ubiquitin ligases RNF126 and Rabring7 regulate endosomal sorting of the epidermal growth factor receptor. J Cell Sci. 2013 Mar 15; 126(Pt 6):1366-80. PMID: 23418353.
      View in: PubMed
    16. Gillis LC, Berry DM, Minden MD, McGlade CJ, Barber DL. Gads (Grb2-related adaptor downstream of Shc) is required for BCR-ABL-mediated lymphoid leukemia. Leukemia. 2013 Aug; 27(8):1666-76. PMID: 23399893; PMCID: PMC4981500.
    17. Krieger JR, Taylor P, Gajadhar AS, Guha A, Moran MF, McGlade CJ. Identification and selected reaction monitoring (SRM) quantification of endocytosis factors associated with Numb. Mol Cell Proteomics. 2013 Feb; 12(2):499-514. PMID: 23211419; PMCID: PMC3567869.
    18. Pham K, Shimoni R, Ludford-Menting MJ, Nowell CJ, Lobachevsky P, Bomzon Z, Gu M, Speed TP, McGlade CJ, Russell SM. Divergent lymphocyte signalling revealed by a powerful new tool for analysis of time-lapse microscopy. Immunol Cell Biol. 2013 Jan; 91(1):70-81. PMID: 23032369.
      View in: PubMed
    19. Wolting CD, Griffiths EK, Sarao R, Prevost BC, Wybenga-Groot LE, McGlade CJ. Biochemical and computational analysis of LNX1 interacting proteins. PLoS One. 2011; 6(11):e26248. PMID: 22087225; PMCID: PMC3210812.
    20. Agnihotri S, Wolf A, Munoz DM, Smith CJ, Gajadhar A, Restrepo A, Clarke ID, Fuller GN, Kesari S, Dirks PB, McGlade CJ, Stanford WL, Aldape K, Mischel PS, Hawkins C, Guha A. A GATA4-regulated tumor suppressor network represses formation of malignant human astrocytomas. J Exp Med. 2011 Apr 11; 208(4):689-702. PMID: 21464220; PMCID: PMC3135351.
    21. Beres BJ, George R, Lougher EJ, Barton M, Verrelli BC, McGlade CJ, Rawls JA, Wilson-Rawls J. Numb regulates Notch1, but not Notch3, during myogenesis. Mech Dev. 2011 May-Jun; 128(5-6):247-57. PMID: 21356309.
      View in: PubMed
    22. Liontos LM, Dissanayake D, Ohashi PS, Weiss A, Dragone LL, McGlade CJ. The Src-like adaptor protein regulates GM-CSFR signaling and monocytic dendritic cell maturation. J Immunol. 2011 Feb 15; 186(4):1923-33. PMID: 21220694.
      View in: PubMed
    23. Lau KM, McGlade CJ. Numb is a negative regulator of HGF dependent cell scattering and Rac1 activation. Exp Cell Res. 2011 Feb 15; 317(4):539-51. PMID: 21147098.
      View in: PubMed
    24. Misquitta-Ali CM, Cheng E, O'Hanlon D, Liu N, McGlade CJ, Tsao MS, Blencowe BJ. Global profiling and molecular characterization of alternative splicing events misregulated in lung cancer. Mol Cell Biol. 2011 Jan; 31(1):138-50. PMID: 21041478; PMCID: PMC3019846.
    25. McGill MA, Dho SE, Weinmaster G, McGlade CJ. Numb regulates post-endocytic trafficking and degradation of Notch1. J Biol Chem. 2009 Sep 25; 284(39):26427-38. PMID: 19567869; PMCID: PMC2785331.
    26. Laprise P, Lau KM, Harris KP, Silva-Gagliardi NF, Paul SM, Beronja S, Beitel GJ, McGlade CJ, Tepass U. Yurt, Coracle, Neurexin IV and the Na(+),K(+)-ATPase form a novel group of epithelial polarity proteins. Nature. 2009 Jun 25; 459(7250):1141-5. PMID: 19553998.
      View in: PubMed
    27. Hughes CE, Auger JM, McGlade J, Eble JA, Pearce AC, Watson SP. Differential roles for the adapters Gads and LAT in platelet activation by GPVI and CLEC-2. J Thromb Haemost. 2008 Dec; 6(12):2152-9. PMID: 18826392; PMCID: PMC2710801.
    28. Pardossi-Piquard R, Chen F, Silva-Gagliardi NF, Szego M, McInnes R, McGlade CJ, St George-Hyslop P, Fraser PE. Overexpression of human CRB1 or related isoforms, CRB2 and CRB3, does not regulate the human presenilin complex in culture cells. Biochemistry. 2007 Dec 04; 46(48):13704-10. PMID: 17988153.
      View in: PubMed
    29. Lee JD, Silva-Gagliardi NF, Tepass U, McGlade CJ, Anderson KV. The FERM protein Epb4.1l5 is required for organization of the neural plate and for the epithelial-mesenchymal transition at the primitive streak of the mouse embryo. Development. 2007 Jun; 134(11):2007-16. PMID: 17507402.
      View in: PubMed
    30. Pakuts B, Debonneville C, Liontos LM, Loreto MP, McGlade CJ. The Src-like adaptor protein 2 regulates colony-stimulating factor-1 receptor signaling and down-regulation. J Biol Chem. 2007 Jun 22; 282(25):17953-63. PMID: 17353186.
      View in: PubMed
    31. Seet BT, Berry DM, Maltzman JS, Shabason J, Raina M, Koretzky GA, McGlade CJ, Pawson T. Efficient T-cell receptor signaling requires a high-affinity interaction between the Gads C-SH3 domain and the SLP-76 RxxK motif. EMBO J. 2007 Feb 07; 26(3):678-89. PMID: 17235283; PMCID: PMC1794392.
    32. Smith CA, Lau KM, Rahmani Z, Dho SE, Brothers G, She YM, Berry DM, Bonneil E, Thibault P, Schweisguth F, Le Borgne R, McGlade CJ. aPKC-mediated phosphorylation regulates asymmetric membrane localization of the cell fate determinant Numb. EMBO J. 2007 Jan 24; 26(2):468-80. PMID: 17203073; PMCID: PMC1783459.
    33. Laprise P, Beronja S, Silva-Gagliardi NF, Pellikka M, Jensen AM, McGlade CJ, Tepass U. The FERM protein Yurt is a negative regulatory component of the Crumbs complex that controls epithelial polarity and apical membrane size. Dev Cell. 2006 Sep; 11(3):363-74. PMID: 16950127.
      View in: PubMed
    34. Wolting C, McGlade CJ, Tritchler D. Cluster analysis of protein array results via similarity of Gene Ontology annotation. BMC Bioinformatics. 2006 Jul 12; 7:338. PMID: 16836750; PMCID: PMC1539024.
    35. Dho SE, Trejo J, Siderovski DP, McGlade CJ. Dynamic regulation of mammalian numb by G protein-coupled receptors and protein kinase C activation: Structural determinants of numb association with the cortical membrane. Mol Biol Cell. 2006 Sep; 17(9):4142-55. PMID: 16837553.
      View in: PubMed
    36. Simoncic PD, McGlade CJ, Tremblay ML. PTP1B and TC-PTP: novel roles in immune-cell signaling. Can J Physiol Pharmacol. 2006 Jul; 84(7):667-75. PMID: 16998530.
      View in: PubMed
    37. Simoncic PD, Bourdeau A, Lee-Loy A, Rohrschneider LR, Tremblay ML, Stanley ER, McGlade CJ. T-cell protein tyrosine phosphatase (Tcptp) is a negative regulator of colony-stimulating factor 1 signaling and macrophage differentiation. Mol Cell Biol. 2006 Jun; 26(11):4149-60. PMID: 16705167; PMCID: PMC1489091.
    38. Young P, Nie J, Wang X, McGlade CJ, Rich MM, Feng G. LNX1 is a perisynaptic Schwann cell specific E3 ubiquitin ligase that interacts with ErbB2. Mol Cell Neurosci. 2005 Oct; 30(2):238-48. PMID: 16122940.
      View in: PubMed
    39. Zhu YX, Benn S, Li ZH, Wei E, Masih-Khan E, Trieu Y, Bali M, McGlade CJ, Claudio JO, Stewart AK. The SH3-SAM adaptor HACS1 is up-regulated in B cell activation signaling cascades. J Exp Med. 2004 Sep 20; 200(6):737-47. PMID: 15381729; PMCID: PMC2211965.
    40. Smith CA, Dho SE, Donaldson J, Tepass U, McGlade CJ. The cell fate determinant numb interacts with EHD/Rme-1 family proteins and has a role in endocytic recycling. Mol Biol Cell. 2004 Aug; 15(8):3698-708. PMID: 15155807.
      View in: PubMed
    41. Nie J, Li SS, McGlade CJ. A novel PTB-PDZ domain interaction mediates isoform-specific ubiquitylation of mammalian Numb. J Biol Chem. 2004 May 14; 279(20):20807-15. PMID: 14990566.
      View in: PubMed
    42. McGill MA, McGlade CJ. Mammalian numb proteins promote Notch1 receptor ubiquitination and degradation of the Notch1 intracellular domain. J Biol Chem. 2003 Jun 20; 278(25):23196-203. PMID: 12682059.
      View in: PubMed
    43. Yamasaki S, Nishida K, Sakuma M, Berry D, McGlade CJ, Hirano T, Saito T. Gads/Grb2-mediated association with LAT is critical for the inhibitory function of Gab2 in T cells. Mol Cell Biol. 2003 Apr; 23(7):2515-29. PMID: 12640133; PMCID: PMC150736.
    44. Salmena L, Lemmers B, Hakem A, Matysiak-Zablocki E, Murakami K, Au PY, Berry DM, Tamblyn L, Shehabeldin A, Migon E, Wakeham A, Bouchard D, Yeh WC, McGlade JC, Ohashi PS, Hakem R. Essential role for caspase 8 in T-cell homeostasis and T-cell-mediated immunity. Genes Dev. 2003 Apr 01; 17(7):883-95. PMID: 12654726; PMCID: PMC196031.
    45. Calderwood DA, Fujioka Y, de Pereda JM, García-Alvarez B, Nakamoto T, Margolis B, McGlade CJ, Liddington RC, Ginsberg MH. Integrin beta cytoplasmic domain interactions with phosphotyrosine-binding domains: a structural prototype for diversity in integrin signaling. Proc Natl Acad Sci U S A. 2003 Mar 04; 100(5):2272-7. PMID: 12606711; PMCID: PMC151330.
    46. Liu Q, Berry D, Nash P, Pawson T, McGlade CJ, Li SS. Structural basis for specific binding of the Gads SH3 domain to an RxxK motif-containing SLP-76 peptide: a novel mode of peptide recognition. Mol Cell. 2003 Feb; 11(2):471-81. PMID: 12620234.
      View in: PubMed
    47. Loreto MP, McGlade CJ. Cloning and characterization of human Src-like adaptor protein 2 and a novel splice isoform, SLAP-2-v. Oncogene. 2003 Jan 16; 22(2):266-73. PMID: 12527895.
      View in: PubMed
    48. Woods SA, McGlade CJ, Guha A. Phosphatidylinositol 3'-kinase and MAPK/ERK kinase 1/2 differentially regulate expression of vascular endothelial growth factor in human malignant astrocytoma cells. Neuro Oncol. 2002 10; 4(4):242-52. PMID: 12356354; PMCID: PMC1920663.
    49. Bunnell SC, Hong DI, Kardon JR, Yamazaki T, McGlade CJ, Barr VA, Samelson LE. T cell receptor ligation induces the formation of dynamically regulated signaling assemblies. J Cell Biol. 2002 Sep 30; 158(7):1263-75. PMID: 12356870; PMCID: PMC2173229.
    50. Berry DM, Nash P, Liu SK, Pawson T, McGlade CJ. A high-affinity Arg-X-X-Lys SH3 binding motif confers specificity for the interaction between Gads and SLP-76 in T cell signaling. Curr Biol. 2002 Aug 06; 12(15):1336-41. PMID: 12176364.
      View in: PubMed
    51. Loreto MP, Berry DM, McGlade CJ. Functional cooperation between c-Cbl and Src-like adaptor protein 2 in the negative regulation of T-cell receptor signaling. Mol Cell Biol. 2002 Jun; 22(12):4241-55. PMID: 12024036; PMCID: PMC133880.
    52. Roncarati R, Sestan N, Scheinfeld MH, Berechid BE, Lopez PA, Meucci O, McGlade JC, Rakic P, D'Adamio L. The gamma-secretase-generated intracellular domain of beta-amyloid precursor protein binds Numb and inhibits Notch signaling. Proc Natl Acad Sci U S A. 2002 May 14; 99(10):7102-7. PMID: 12011466; PMCID: PMC124535.
    53. French MB, Koch U, Shaye RE, McGill MA, Dho SE, Guidos CJ, McGlade CJ. Transgenic expression of numb inhibits notch signaling in immature thymocytes but does not alter T cell fate specification. J Immunol. 2002 Apr 01; 168(7):3173-80. PMID: 11907069.
      View in: PubMed
    54. Cheng A, Uetani N, Simoncic PD, Chaubey VP, Lee-Loy A, McGlade CJ, Kennedy BP, Tremblay ML. Attenuation of leptin action and regulation of obesity by protein tyrosine phosphatase 1B. Dev Cell. 2002 Apr; 2(4):497-503. PMID: 11970899.
      View in: PubMed
    55. Simoncic PD, Lee-Loy A, Barber DL, Tremblay ML, McGlade CJ. The T cell protein tyrosine phosphatase is a negative regulator of janus family kinases 1 and 3. Curr Biol. 2002 Mar 19; 12(6):446-53. PMID: 11909529.
      View in: PubMed
    56. Pellikka M, Tanentzapf G, Pinto M, Smith C, McGlade CJ, Ready DF, Tepass U. Crumbs, the Drosophila homologue of human CRB1/RP12, is essential for photoreceptor morphogenesis. Nature. 2002 Mar 14; 416(6877):143-9. PMID: 11850625.
      View in: PubMed
    57. Nie J, McGill MA, Dermer M, Dho SE, Wolting CD, McGlade CJ. LNX functions as a RING type E3 ubiquitin ligase that targets the cell fate determinant Numb for ubiquitin-dependent degradation. EMBO J. 2002 Jan 15; 21(1-2):93-102. PMID: 11782429; PMCID: PMC125803.
    58. Yeh E, Dermer M, Commisso C, Zhou L, McGlade CJ, Boulianne GL. Neuralized functions as an E3 ubiquitin ligase during Drosophila development. Curr Biol. 2001 Oct 30; 11(21):1675-9. PMID: 11696324.
      View in: PubMed
    59. Liu SK, Berry DM, McGlade CJ. The role of Gads in hematopoietic cell signalling. Oncogene. 2001 Oct 01; 20(44):6284-90. PMID: 11607830.
      View in: PubMed
    60. Claudio JO, Zhu YX, Benn SJ, Shukla AH, McGlade CJ, Falcioni N, Stewart AK. HACS1 encodes a novel SH3-SAM adaptor protein differentially expressed in normal and malignant hematopoietic cells. Oncogene. 2001 Aug 30; 20(38):5373-7. PMID: 11536050.
      View in: PubMed
    61. Yoder J, Pham C, Iizuka YM, Kanagawa O, Liu SK, McGlade J, Cheng AM. Requirement for the SLP-76 adaptor GADS in T cell development. Science. 2001 Mar 09; 291(5510):1987-91. PMID: 11239162.
      View in: PubMed
    62. Berry DM, Benn SJ, Cheng AM, McGlade CJ. Caspase-dependent cleavage of the hematopoietic specific adaptor protein Gads alters signalling from the T cell receptor. Oncogene. 2001 Mar 08; 20(10):1203-11. PMID: 11313864.
      View in: PubMed
    63. Tanentzapf G, Smith C, McGlade J, Tepass U. Apical, lateral, and basal polarization cues contribute to the development of the follicular epithelium during Drosophila oogenesis. J Cell Biol. 2000 Nov 13; 151(4):891-904. PMID: 11076972.
      View in: PubMed
    64. Asazuma N, Wilde JI, Berlanga O, Leduc M, Leo A, Schweighoffer E, Tybulewicz V, Bon C, Liu SK, McGlade CJ, Schraven B, Watson SP. Interaction of linker for activation of T cells with multiple adapter proteins in platelets activated by the glycoprotein VI-selective ligand, convulxin. J Biol Chem. 2000 Oct 27; 275(43):33427-34. PMID: 10942756.
      View in: PubMed
    65. Liu SK, Smith CA, Arnold R, Kiefer F, McGlade CJ. The adaptor protein Gads (Grb2-related adaptor downstream of Shc) is implicated in coupling hemopoietic progenitor kinase-1 to the activated TCR. J Immunol. 2000 Aug 01; 165(3):1417-26. PMID: 10903746.
      View in: PubMed
    66. Zhang W, Trible RP, Zhu M, Liu SK, McGlade CJ, Samelson LE. Association of Grb2, Gads, and phospholipase C-gamma 1 with phosphorylated LAT tyrosine residues. Effect of LAT tyrosine mutations on T cell angigen receptor-mediated signaling. J Biol Chem. 2000 Jul 28; 275(30):23355-61. PMID: 10811803.
      View in: PubMed
    67. Dho SE, French MB, Woods SA, McGlade CJ. Characterization of four mammalian numb protein isoforms. Identification of cytoplasmic and membrane-associated variants of the phosphotyrosine binding domain. J Biol Chem. 1999 Nov 12; 274(46):33097-104. PMID: 10551880.
      View in: PubMed
    68. Schmandt R, Liu SK, McGlade CJ. Cloning and characterization of mPAL, a novel Shc SH2 domain-binding protein expressed in proliferating cells. Oncogene. 1999 Mar 11; 18(10):1867-79. PMID: 10086341.
      View in: PubMed
    69. Liu SK, Fang N, Koretzky GA, McGlade CJ. The hematopoietic-specific adaptor protein gads functions in T-cell signaling via interactions with the SLP-76 and LAT adaptors. Curr Biol. 1999 Jan 28; 9(2):67-75. PMID: 10021361.
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    70. Liu SK, McGlade CJ. Gads is a novel SH2 and SH3 domain-containing adaptor protein that binds to tyrosine-phosphorylated Shc. Oncogene. 1998 Dec 17; 17(24):3073-82. PMID: 9872323.
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    71. Li SC, Zwahlen C, Vincent SJ, McGlade CJ, Kay LE, Pawson T, Forman-Kay JD. Structure of a Numb PTB domain-peptide complex suggests a basis for diverse binding specificity. Nat Struct Biol. 1998 Dec; 5(12):1075-83. PMID: 9846878.
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    72. Wolting CD, McGlade CJ. Cloning and chromosomal localization of a new member of the bHLH/PAS transcription factor family. Mamm Genome. 1998 Jun; 9(6):463-8. PMID: 9585435.
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    73. Dho SE, Jacob S, Wolting CD, French MB, Rohrschneider LR, McGlade CJ. The mammalian numb phosphotyrosine-binding domain. Characterization of binding specificity and identification of a novel PDZ domain-containing numb binding protein, LNX. J Biol Chem. 1998 Apr 10; 273(15):9179-87. PMID: 9535908.
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    74. Roche S, McGlade J, Jones M, Gish GD, Pawson T, Courtneidge SA. Requirement of phospholipase C gamma, the tyrosine phosphatase Syp and the adaptor proteins Shc and Nck for PDGF-induced DNA synthesis: evidence for the existence of Ras-dependent and Ras-independent pathways. EMBO J. 1996 Sep 16; 15(18):4940-8. PMID: 8890167; PMCID: PMC452231.
    75. Verdi JM, Schmandt R, Bashirullah A, Jacob S, Salvino R, Craig CG, Program AE, Lipshitz HD, McGlade CJ. Mammalian NUMB is an evolutionarily conserved signaling adapter protein that specifies cell fate. Curr Biol. 1996 Sep 01; 6(9):1134-45. PMID: 8805372.
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    76. Garrity PA, Rao Y, Salecker I, McGlade J, Pawson T, Zipursky SL. Drosophila photoreceptor axon guidance and targeting requires the dreadlocks SH2/SH3 adapter protein. Cell. 1996 May 31; 85(5):639-50. PMID: 8646773.
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    77. Staub O, Dho S, Henry P, Correa J, Ishikawa T, McGlade J, Rotin D. WW domains of Nedd4 bind to the proline-rich PY motifs in the epithelial Na+ channel deleted in Liddle's syndrome. EMBO J. 1996 May 15; 15(10):2371-80. PMID: 8665844; PMCID: PMC450167.
    78. Charest A, Wagner J, Jacob S, McGlade CJ, Tremblay ML. Phosphotyrosine-independent binding of SHC to the NPLH sequence of murine protein-tyrosine phosphatase-PEST. Evidence for extended phosphotyrosine binding/phosphotyrosine interaction domain recognition specificity. J Biol Chem. 1996 Apr 05; 271(14):8424-9. PMID: 8626541.
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    79. Lai KM, Olivier JP, Gish GD, Henkemeyer M, McGlade J, Pawson T. A Drosophila shc gene product is implicated in signaling by the DER receptor tyrosine kinase. Mol Cell Biol. 1995 Sep; 15(9):4810-8. PMID: 7651398; PMCID: PMC230725.
    80. Salcini AE, McGlade J, Pelicci G, Nicoletti I, Pawson T, Pelicci PG. Formation of Shc-Grb2 complexes is necessary to induce neoplastic transformation by overexpression of Shc proteins. Oncogene. 1994 Oct; 9(10):2827-36. PMID: 8084588.
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    81. Yokote K, Mori S, Hansen K, McGlade J, Pawson T, Heldin CH, Claesson-Welsh L. Direct interaction between Shc and the platelet-derived growth factor beta-receptor. J Biol Chem. 1994 May 27; 269(21):15337-43. PMID: 8195171.
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    82. Meyer S, LaBudda K, McGlade J, Hayman MJ. Analysis of the role of the Shc and Grb2 proteins in signal transduction by the v-ErbB protein. Mol Cell Biol. 1994 May; 14(5):3253-62. PMID: 7909355; PMCID: PMC358692.
    83. Henkemeyer M, Marengere LE, McGlade J, Olivier JP, Conlon RA, Holmyard DP, Letwin K, Pawson T. Immunolocalization of the Nuk receptor tyrosine kinase suggests roles in segmental patterning of the brain and axonogenesis. Oncogene. 1994 Apr; 9(4):1001-14. PMID: 8134103.
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    84. Songyang Z, Shoelson SE, McGlade J, Olivier P, Pawson T, Bustelo XR, Barbacid M, Sabe H, Hanafusa H, Yi T, et al. Specific motifs recognized by the SH2 domains of Csk, 3BP2, fps/fes, GRB-2, HCP, SHC, Syk, and Vav. Mol Cell Biol. 1994 Apr; 14(4):2777-85. PMID: 7511210; PMCID: PMC358643.
    85. Crowe AJ, McGlade J, Pawson T, Hayman MJ. Phosphorylation of the SHC proteins on tyrosine correlates with the transformation of fibroblasts and erythroblasts by the v-sea tyrosine kinase. Oncogene. 1994 Feb; 9(2):537-44. PMID: 8290264.
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    86. McGlade J, Brunkhorst B, Anderson D, Mbamalu G, Settleman J, Dedhar S, Rozakis-Adcock M, Chen LB, Pawson T. The N-terminal region of GAP regulates cytoskeletal structure and cell adhesion. EMBO J. 1993 Aug; 12(8):3073-81. PMID: 8344248; PMCID: PMC413572.
    87. Segatto O, Pelicci G, Giuli S, Digiesi G, Di Fiore PP, McGlade J, Pawson T, Pelicci PG. Shc products are substrates of erbB-2 kinase. Oncogene. 1993 Aug; 8(8):2105-12. PMID: 8101647.
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    88. Pawson T, Olivier P, Rozakis-Adcock M, McGlade J, Henkemeyer M. Proteins with SH2 and SH3 domains couple receptor tyrosine kinases to intracellular signalling pathways. Philos Trans R Soc Lond B Biol Sci. 1993 Jun 29; 340(1293):279-85. PMID: 8103930.
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    89. Ruff-Jamison S, McGlade J, Pawson T, Chen K, Cohen S. Epidermal growth factor stimulates the tyrosine phosphorylation of SHC in the mouse. J Biol Chem. 1993 Apr 15; 268(11):7610-2. PMID: 7681824.
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    90. Pronk GJ, McGlade J, Pelicci G, Pawson T, Bos JL. Insulin-induced phosphorylation of the 46- and 52-kDa Shc proteins. J Biol Chem. 1993 Mar 15; 268(8):5748-53. PMID: 8449939.
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    91. Rozakis-Adcock M, McGlade J, Mbamalu G, Pelicci G, Daly R, Li W, Batzer A, Thomas S, Brugge J, Pelicci PG, Schlessinger J, Pawson T, et al. Association of the Shc and Grb2/Sem5 SH2-containing proteins is implicated in activation of the Ras pathway by tyrosine kinases. Nature. 1992 Dec 17; 360(6405):689-92. PMID: 1465135.
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    92. McGlade J, Cheng A, Pelicci G, Pelicci PG, Pawson T. Shc proteins are phosphorylated and regulated by the v-Src and v-Fps protein-tyrosine kinases. Proc Natl Acad Sci U S A. 1992 Oct 01; 89(19):8869-73. PMID: 1409579; PMCID: PMC50025.
    93. Pelicci G, Lanfrancone L, Grignani F, McGlade J, Cavallo F, Forni G, Nicoletti I, Grignani F, Pawson T, Pelicci PG. A novel transforming protein (SHC) with an SH2 domain is implicated in mitogenic signal transduction. Cell. 1992 Jul 10; 70(1):93-104. PMID: 1623525.
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    94. McGlade CJ, Ellis C, Reedijk M, Anderson D, Mbamalu G, Reith AD, Panayotou G, End P, Bernstein A, Kazlauskas A, et al. SH2 domains of the p85 alpha subunit of phosphatidylinositol 3-kinase regulate binding to growth factor receptors. Mol Cell Biol. 1992 Mar; 12(3):991-7. PMID: 1372092; PMCID: PMC369531.
    95. McLorie W, McGlade CJ, Takayesu D, Branton PE. Individual adenovirus E1B proteins induce transformation independently but by additive pathways. J Gen Virol. 1991 Jun; 72 ( Pt 6):1467-71. PMID: 1828498.
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    96. McGlade CJ, Tremblay ML, Branton PE. Mapping of a phosphorylation site in the 176R (19 kDa) early region 1B protein of human adenovirus type 5. Virology. 1989 Jan; 168(1):119-27. PMID: 2521265.
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    97. McGlade CJ, Tremblay ML, Yee SP, Ross R, Branton PE. Acylation of the 176R (19-kilodalton) early region 1B protein of human adenovirus type 5. J Virol. 1987 Oct; 61(10):3227-34. PMID: 2957509; PMCID: PMC255902.
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