Loading...
Reach Banner
Keywords
Last name
Institution

John Rubinstein

TitleSenior Scientist
InstitutionThe Hospital for Sick Children
DepartmentMolecular Medicine
Address555 University Avenue
Toronto ON M5G 1X8
Phone416-813-1500
ORCID ORCID Icon0000-0003-0566-2209 Additional info
vCardDownload vCard

    Collapse Biography 
    Collapse education and training
    University of Cambridge, United KingdomPhD2001MRC Laboratory of Molecular Biology & MRC Mitochondrial Biology Unit
    University of Guelph, CanadaBSc1998Physical and Engineering Science
    Collapse awards and honors
    2014 - 2021Electron cryomicroscopy, Canada Research Chair (Tier 1)
    2014GE Healthcare New Investigator Award, Canadian Society for Molecular Biosciences
    2013Burton Medal (for outstanding achievement by a scientist under the age of 40), Microscopy Society of America
    2011Audience’s Choice Seminar Award, University of Toronto, Medical Biophysics Retreat
    2009 - 2014Early Researcher Award, Ontario Ministry of Research and Innovation
    2007 - 2012New Investigator Award, CIHR
    2003 - 2005Postdoctoral Fellowship, National Cancer Institute of Canada
    1998 - 2001Landmark Graduate Award, Huntington Disease Society of Canada
    1998 - 2001Scholarship, Cambridge Commonwealth Trust
    1998 - 2001United Kingdom, Overseas Research Scholarships
    1998 - 2000Raymond and Beverly Sackler Fellowship, University of Cambridge
    1994 - 1998Canada Scholarship
    1998College of Physical & Engineering Science Graduation Prize, University of Guelph

    Collapse Overview 
    Collapse overview
    John Rubinstein obtained his B.Sc from the University of Guelph in 1998. He received his PhD from Cambridge University (2002) where he worked in Medical Research Council laboratories under the supervision of Sir John E. Walker (Nobel Prize for Chemistry, 1997) and Dr. Richard Henderson (Nobel Prize for Chemistry, 2017). Dr. Rubinstein was a Post-doctoral Research Associate at the MRC Laboratory of Molecular Biology before returning to Canada for a National Cancer Institute of Canada (NCIC) post-doctoral fellowship at the Banting and Best Department of Medical Research. He joined the Research Institute of The Hospital for Sick Children in 2006.


    Collapse Research 
    Collapse research activities and funding
    MOP 81249     (M. Cygler)Oct 1, 2006 - Sep 30, 2009
    CIHR
    Structural and functional studies of the inner membrane machinery of tonB-dependent transport
    Role: Co-applicant

    MOP 81294     (John Rubinstein)Oct 1, 2006 - Sep 30, 2018
    CIHR - Operating Grant
    Structural Analysis of the ATP synthase and V-ATPase
    Role: Principal Applicant

         (John Rubinstein)Nov 1, 2006
    CFI Leaders Opportunity Fund/ORF
    Electron cryomicroscopy of macromolecular assemblies

         (John Rubinstein)Apr 1, 2007 - Mar 31, 2012
    CIHR
    New Investigator Award

         (John Rubinstein)May 1, 2009 - Apr 30, 2014
    Ontario Ministry of Research and Innovation
    Early Researcher Award

         (John Rubinstein)Apr 1, 2012 - Mar 31, 2017
    NSERC - Discovery
    High-resolution single particle electron cryomicroscopy of membrane protein complexes
    Role: Principal Applicant

         (John Rubinstein)Apr 1, 2014 - Mar 31, 2021
    CIHR - Canada Research Chair (Tier 1)
    Electron cryomicroscopy
    Role: Principal Applicant

         (John Rubinstein)Apr 1, 2017 - Mar 31, 2022
    NSERC - Discovery Grant
    Cryo-EM of dynamic protein complexes in protein quality control
    Role: Principal Applicant

         (John Rubinstein)Jan 1, 2018
    Canada Foundation for Innovation and Ontario Research Fund
    Resource for high-resolution high-throughput biomolecular cryo-EM (Infrastructure only, no funds for personal research)
    Role: Principal Applicant

    Collapse Bibliographic 
    Collapse selected publications
    Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Faculty can login to make corrections and additions.
    List All   |   Timeline
    1. Huang R, Ripstein ZA, Rubinstein JL, Kay LE. Cooperative subunit dynamics modulate p97 function. Proc Natl Acad Sci U S A. 2019 Jan 02; 116(1):158-167. PMID: 30584095.
      View in: PubMed
    2. Wiseman B, Nitharwal RG, Fedotovskaya O, Schäfer J, Guo H, Kuang Q, Benlekbir S, Sjöstrand D, Ädelroth P, Rubinstein JL, Brzezinski P, Högbom M. Structure of a functional obligate complex III2IV2 respiratory supercomplex from Mycobacterium smegmatis. Nat Struct Mol Biol. 2018 Dec; 25(12):1128-1136. PMID: 30518849.
      View in: PubMed
    3. Vahidi S, Ripstein ZA, Bonomi M, Yuwen T, Mabanglo MF, Juravsky JB, Rizzolo K, Velyvis A, Houry WA, Vendruscolo M, Rubinstein JL, Kay LE. Reversible inhibition of the ClpP protease via an N-terminal conformational switch. Proc Natl Acad Sci U S A. 2018 07 10; 115(28):E6447-E6456. PMID: 29941580.
      View in: PubMed
    4. Abbas YM, Toye AM, Rubinstein JL, Reithmeier RAF. Band 3 function and dysfunction in a structural context. Curr Opin Hematol. 2018 05; 25(3):163-170. PMID: 29438259.
      View in: PubMed
    5. Sun C, Benlekbir S, Venkatakrishnan P, Wang Y, Hong S, Hosler J, Tajkhorshid E, Rubinstein JL, Gennis RB. Structure of the alternative complex III in a supercomplex with cytochrome oxidase. Nature. 2018 05; 557(7703):123-126. PMID: 29695868.
      View in: PubMed
    6. Venkatakrishnan P, Benlekbir S, Sun C, Hong S, Hosler J, Rubinstein J, Gennis RB. WITHDRAWN: Characterization of the supercomplex formed by the alternative complex III and the terminal aa3 oxidase from Flavobacterium johnsoniae isolated in styrene:maleic acid copolymer nanodiscs. Biochim Biophys Acta Bioenerg. 2018 Feb 12. PMID: 29408430.
      View in: PubMed
    7. Guo H, Bueler SA, Rubinstein JL. Atomic model for the dimeric FO region of mitochondrial ATP synthase. Science. 2017 11 17; 358(6365):936-940. PMID: 29074581.
      View in: PubMed
    8. Ereño-Orbea J, Sicard T, Cui H, Mazhab-Jafari MT, Benlekbir S, Guarné A, Rubinstein JL, Julien JP. Molecular basis of human CD22 function and therapeutic targeting. Nat Commun. 2017 10 02; 8(1):764. PMID: 28970495.
      View in: PubMed
    9. Zhao J, Beyrakhova K, Liu Y, Alvarez CP, Bueler SA, Xu L, Xu C, Boniecki MT, Kanelis V, Luo ZQ, Cygler M, Rubinstein JL. Molecular basis for the binding and modulation of V-ATPase by a bacterial effector protein. PLoS Pathog. 2017 Jun; 13(6):e1006394. PMID: 28570695.
      View in: PubMed
    10. Kitevski-LeBlanc J, Fradet-Turcotte A, Kukic P, Wilson MD, Portella G, Yuwen T, Panier S, Duan S, Canny MD, van Ingen H, Arrowsmith CH, Rubinstein JL, Vendruscolo M, Durocher D, Kay LE. The RNF168 paralog RNF169 defines a new class of ubiquitylated histone reader involved in the response to DNA damage. Elife. 2017 04 13; 6. PMID: 28406400.
      View in: PubMed
    11. Ripstein ZA, Huang R, Augustyniak R, Kay LE, Rubinstein JL. Structure of a AAA+ unfoldase in the process of unfolding substrate. Elife. 2017 04 08; 6. PMID: 28390173.
      View in: PubMed
    12. Punjani A, Rubinstein JL, Fleet DJ, Brubaker MA. cryoSPARC: algorithms for rapid unsupervised cryo-EM structure determination. Nat Methods. 2017 03; 14(3):290-296. PMID: 28165473.
      View in: PubMed
    13. Rubinstein JL. Cryo-EM Captures the Dynamics of Ion Channel Opening. Cell. 2017 01 26; 168(3):341-343. PMID: 28129535.
      View in: PubMed
    14. Mazhab-Jafari MT, Rohou A, Schmidt C, Bueler SA, Benlekbir S, Robinson CV, Rubinstein JL. Atomic model for the membrane-embedded VO motor of a eukaryotic V-ATPase. Nature. 2016 11 03; 539(7627):118-122. PMID: 27776355.
      View in: PubMed
    15. Koo J, Lamers RP, Rubinstein JL, Burrows LL, Howell PL. Structure of the Pseudomonas aeruginosa Type IVa Pilus Secretin at 7.4 Å. Structure. 2016 Oct 04; 24(10):1778-1787. PMID: 27705815.
      View in: PubMed
    16. Ni X, Davis JH, Jain N, Razi A, Benlekbir S, McArthur AG, Rubinstein JL, Britton RA, Williamson JR, Ortega J. YphC and YsxC GTPases assist the maturation of the central protuberance, GTPase associated region and functional core of the 50S ribosomal subunit. Nucleic Acids Res. 2016 09 30; 44(17):8442-55. PMID: 27484475.
      View in: PubMed
    17. Wilson MD, Benlekbir S, Fradet-Turcotte A, Sherker A, Julien JP, McEwan A, Noordermeer SM, Sicheri F, Rubinstein JL, Durocher D. The structural basis of modified nucleosome recognition by 53BP1. Nature. 2016 08 04; 536(7614):100-3. PMID: 27462807.
      View in: PubMed
    18. Mazhab-Jafari MT, Rubinstein JL. Cryo-EM studies of the structure and dynamics of vacuolar-type ATPases. Sci Adv. 2016 07; 2(7):e1600725. PMID: 27532044.
      View in: PubMed
    19. Huang R, Ripstein ZA, Augustyniak R, Lazniewski M, Ginalski K, Kay LE, Rubinstein JL. Unfolding the mechanism of the AAA+ unfoldase VAT by a combined cryo-EM, solution NMR study. Proc Natl Acad Sci U S A. 2016 07 19; 113(29):E4190-9. PMID: 27402735; PMCID: PMC4961139 [Available on 01/19/17].
    20. Ripstein ZA, Rubinstein JL. Processing of Cryo-EM Movie Data. Methods Enzymol. 2016; 579:103-24. PMID: 27572725.
      View in: PubMed
    21. Schep DG, Zhao J, Rubinstein JL. Models for the a subunits of the Thermus thermophilus V/A-ATPase and Saccharomyces cerevisiae V-ATPase enzymes by cryo-EM and evolutionary covariance. Proc Natl Acad Sci U S A. 2016 Mar 22; 113(12):3245-50. PMID: 26951669; PMCID: PMC4812769 [Available on 09/22/16].
    22. Rosenthal PB, Rubinstein JL. Validating maps from single particle electron cryomicroscopy. Curr Opin Struct Biol. 2015 Oct; 34:135-44. PMID: 26605834.
      View in: PubMed
    23. Zhou A, Rohou A, Schep DG, Bason JV, Montgomery MG, Walker JE, Grigorieff N, Rubinstein JL. Structure and conformational states of the bovine mitochondrial ATP synthase by cryo-EM. Elife. 2015 Oct 06; 4:e10180. PMID: 26439008.
      View in: PubMed
    24. Pimentel-Elardo SM, Sørensen D, Ho L, Ziko M, Bueler SA, Lu S, Tao J, Moser A, Lee R, Agard D, Fairn G, Rubinstein JL, Shoichet BK, Nodwell JR. Activity-Independent Discovery of Secondary Metabolites Using Chemical Elicitation and Cheminformatic Inference. ACS Chem Biol. 2015 Nov 20; 10(11):2616-23. PMID: 26352211; PMCID: PMC4658348 [Available on 11/20/16].
    25. Ji AX, Chu A, Nielsen TK, Benlekbir S, Rubinstein JL, Privé GG. Structural Insights into KCTD Protein Assembly and Cullin3 Recognition. J Mol Biol. 2016 Jan 16; 428(1):92-107. PMID: 26334369.
      View in: PubMed
    26. Rubinstein JL, Brubaker MA. Alignment of cryo-EM movies of individual particles by optimization of image translations. J Struct Biol. 2015 Nov; 192(2):188-95. PMID: 26296328.
      View in: PubMed
    27. Zhao J, Brubaker MA, Benlekbir S, Rubinstein JL. Description and comparison of algorithms for correcting anisotropic magnification in cryo-EM images. J Struct Biol. 2015 Nov; 192(2):209-15. PMID: 26087140.
      View in: PubMed
    28. Zhao J, Benlekbir S, Rubinstein JL. Electron cryomicroscopy observation of rotational states in a eukaryotic V-ATPase. Nature. 2015 May 14; 521(7551):241-5. PMID: 25971514.
      View in: PubMed
    29. Bueler SA, Rubinstein JL. Vma9p need not be associated with the yeast V-ATPase for fully-coupled proton pumping activity in vitro. Biochemistry. 2015 Jan 27; 54(3):853-8. PMID: 25546637.
      View in: PubMed
    30. Zhao J, Rubinstein JL. The study of vacuolar-type ATPases by single particle electron microscopy. Biochem Cell Biol. 2014 Dec; 92(6):460-6. PMID: 25350640.
      View in: PubMed
    31. Smith MT, Rubinstein JL. Structural biology. Beyond blob-ology. Science. 2014 Aug 08; 345(6197):617-9. PMID: 25104368.
      View in: PubMed
    32. Langlois R, Pallesen J, Ash JT, Nam Ho D, Rubinstein JL, Frank J. Automated particle picking for low-contrast macromolecules in cryo-electron microscopy. J Struct Biol. 2014 Apr; 186(1):1-7. PMID: 24607413; PMCID: PMC4063204.
    33. Zhao J, Rubinstein JL. Enzyme mechanisms: Flexibility leads to function. Nat Chem. 2014 Mar; 6(3):170-1. PMID: 24557126.
      View in: PubMed
    34. Marshansky V, Rubinstein JL, Grüber G. Eukaryotic V-ATPase: novel structural findings and functional insights. Biochim Biophys Acta. 2014 Jun; 1837(6):857-79. PMID: 24508215.
      View in: PubMed
    35. Marr CR, Benlekbir S, Rubinstein JL. Fabrication of carbon films with ~ 500nm holes for cryo-EM with a direct detector device. J Struct Biol. 2014 Jan; 185(1):42-7. PMID: 24269484.
      View in: PubMed
    36. Ishiyama N, Tanaka N, Abe K, Yang YJ, Abbas YM, Umitsu M, Nagar B, Bueler SA, Rubinstein JL, Takeichi M, Ikura M. An autoinhibited structure of a-catenin and its implications for vinculin recruitment to adherens junctions. J Biol Chem. 2013 May 31; 288(22):15913-25. PMID: 23589308.
      View in: PubMed
    37. Zhao J, Brubaker MA, Rubinstein JL. TMaCS: a hybrid template matching and classification system for partially-automated particle selection. J Struct Biol. 2013 Mar; 181(3):234-42. PMID: 23333657.
      View in: PubMed
    38. Hosokawa H, Dip PV, Merkulova M, Bakulina A, Zhuang Z, Khatri A, Jian X, Keating SM, Bueler SA, Rubinstein JL, Randazzo PA, Ausiello DA, Grüber G, Marshansky V. The N termini of a-subunit isoforms are involved in signaling between vacuolar H+-ATPase (V-ATPase) and cytohesin-2. J Biol Chem. 2013 Feb 22; 288(8):5896-913. PMID: 23288846; PMCID: PMC3581410.
    39. Lau WC, Rubinstein JL. Single particle electron microscopy. Methods Mol Biol. 2013; 955:401-26. PMID: 23132073.
      View in: PubMed
    40. Benlekbir S, Bueler SA, Rubinstein JL. Structure of the vacuolar-type ATPase from Saccharomyces cerevisiae at 11-Å resolution. Nat Struct Mol Biol. 2012 Dec; 19(12):1356-62. PMID: 23142977.
      View in: PubMed
    41. Baker LA, Watt IN, Runswick MJ, Walker JE, Rubinstein JL. Arrangement of subunits in intact mammalian mitochondrial ATP synthase determined by cryo-EM. Proc Natl Acad Sci U S A. 2012 Jul 17; 109(29):11675-80. PMID: 22753497; PMCID: PMC3406826.
    42. Errington WJ, Khan MQ, Bueler SA, Rubinstein JL, Chakrabartty A, Privé GG. Adaptor protein self-assembly drives the control of a cullin-RING ubiquitin ligase. Structure. 2012 Jul 03; 20(7):1141-53. PMID: 22632832.
      View in: PubMed
    43. Lau WC, Rubinstein JL. Subnanometre-resolution structure of the intact Thermus thermophilus H+-driven ATP synthase. Nature. 2011 Dec 18; 481(7380):214-8. PMID: 22178924.
      View in: PubMed
    44. Baldwin AJ, Lioe H, Hilton GR, Baker LA, Rubinstein JL, Kay LE, Benesch JL. The polydispersity of aB-crystallin is rationalized by an interconverting polyhedral architecture. Structure. 2011 Dec 07; 19(12):1855-63. PMID: 22153508.
      View in: PubMed
    45. Henderson R, Chen S, Chen JZ, Grigorieff N, Passmore LA, Ciccarelli L, Rubinstein JL, Crowther RA, Stewart PL, Rosenthal PB. Tilt-pair analysis of images from a range of different specimens in single-particle electron cryomicroscopy. J Mol Biol. 2011 Nov 11; 413(5):1028-46. PMID: 21939668; PMCID: PMC3220764.
    46. Baker LA, Rubinstein JL. Edged watershed segmentation: a semi-interactive algorithm for segmentation of low-resolution maps from electron cryomicroscopy. J Struct Biol. 2011 Oct; 176(1):127-32. PMID: 21763426.
      View in: PubMed
    47. Lovell JF, Jin CS, Huynh E, Jin H, Kim C, Rubinstein JL, Chan WC, Cao W, Wang LV, Zheng G. Porphysome nanovesicles generated by porphyrin bilayers for use as multimodal biophotonic contrast agents. Nat Mater. 2011 Apr; 10(4):324-32. PMID: 21423187.
      View in: PubMed
    48. Jaitly N, Brubaker MA, Rubinstein JL, Lilien RH. A Bayesian method for 3D macromolecular structure inference using class average images from single particle electron microscopy. Bioinformatics. 2010 Oct 01; 26(19):2406-15. PMID: 20702397.
      View in: PubMed
    49. Cardarelli L, Pell LG, Neudecker P, Pirani N, Liu A, Baker LA, Rubinstein JL, Maxwell KL, Davidson AR. Phages have adapted the same protein fold to fulfill multiple functions in virion assembly. Proc Natl Acad Sci U S A. 2010 Aug 10; 107(32):14384-9. PMID: 20660769; PMCID: PMC2922521.
    50. Schmidt-Krey I, Rubinstein JL. Electron cryomicroscopy of membrane proteins: specimen preparation for two-dimensional crystals and single particles. Micron. 2011 Feb; 42(2):107-16. PMID: 20678942; PMCID: PMC2995848.
    51. MacKinnon N, Guérin G, Liu B, Gradinaru CC, Rubinstein JL, Macdonald PM. Triggered instability of liposomes bound to hydrophobically modified core-shell PNIPAM hydrogel beads. Langmuir. 2010 Jan 19; 26(2):1081-9. PMID: 19754070.
      View in: PubMed
    52. Lau WC, Rubinstein JL. Structure of intact Thermus thermophilus V-ATPase by cryo-EM reveals organization of the membrane-bound V(O) motor. Proc Natl Acad Sci U S A. 2010 Jan 26; 107(4):1367-72. PMID: 20080582.
      View in: PubMed
    53. Baker LA, Rubinstein JL. Radiation damage in electron cryomicroscopy. Methods Enzymol. 2010; 481:371-88. PMID: 20887865.
      View in: PubMed
    54. Baker LA, Smith EA, Bueler SA, Rubinstein JL. The resolution dependence of optimal exposures in liquid nitrogen temperature electron cryomicroscopy of catalase crystals. J Struct Biol. 2010 Mar; 169(3):431-7. PMID: 19958834.
      View in: PubMed
    55. Cardarelli L, Lam R, Tuite A, Baker LA, Sadowski PD, Radford DR, Rubinstein JL, Battaile KP, Chirgadze N, Maxwell KL, Davidson AR. The crystal structure of bacteriophage HK97 gp6: defining a large family of head-tail connector proteins. J Mol Biol. 2010 Jan 29; 395(4):754-68. PMID: 19895817.
      View in: PubMed
    56. Rujiviphat J, Meglei G, Rubinstein JL, McQuibban GA. Phospholipid association is essential for dynamin-related protein Mgm1 to function in mitochondrial membrane fusion. J Biol Chem. 2009 Oct 16; 284(42):28682-6. PMID: 19703904; PMCID: PMC2781412.
    57. Bueler SA, Rubinstein JL. Location of subunit d in the peripheral stalk of the ATP synthase from Saccharomyces cerevisiae. Biochemistry. 2008 Nov 11; 47(45):11804-10. PMID: 18937496.
      View in: PubMed
    58. Lau WC, Baker LA, Rubinstein JL. Cryo-EM structure of the yeast ATP synthase. J Mol Biol. 2008 Oct 24; 382(5):1256-64. PMID: 18722382.
      View in: PubMed
    59. Sprangers R, Li X, Mao X, Rubinstein JL, Schimmer AD, Kay LE. TROSY-based NMR evidence for a novel class of 20S proteasome inhibitors. Biochemistry. 2008 Jul 01; 47(26):6727-34. PMID: 18540636.
      View in: PubMed
    60. Tocilj A, Munger C, Proteau A, Morona R, Purins L, Ajamian E, Wagner J, Papadopoulos M, Van Den Bosch L, Rubinstein JL, Féthière J, Matte A, Cygler M. Bacterial polysaccharide co-polymerases share a common framework for control of polymer length. Nat Struct Mol Biol. 2008 Feb; 15(2):130-8. PMID: 18204465.
      View in: PubMed
    61. Baker LA, Rubinstein JL. Angle determination for side views in single particle electron microscopy. J Struct Biol. 2008 May; 162(2):260-70. PMID: 18272396.
      View in: PubMed
    62. Rubinstein JL. Structural analysis of membrane protein complexes by single particle electron microscopy. Methods. 2007 Apr; 41(4):409-16. PMID: 17367713.
      View in: PubMed
    63. Rubinstein JL, Dickson VK, Runswick MJ, Walker JE. ATP synthase from Saccharomyces cerevisiae: location of subunit h in the peripheral stalk region. J Mol Biol. 2005 Jan 21; 345(3):513-20. PMID: 15581895.
      View in: PubMed
    64. Rubinstein JL, Walker JE, Henderson R. Structure of the mitochondrial ATP synthase by electron cryomicroscopy. EMBO J. 2003 Dec 01; 22(23):6182-92. PMID: 14633978.
      View in: PubMed
    65. Rubinstein JL, Holt LJ, Walker JE, Tomlinson IM. Use of phage display and high-density screening for the isolation of an antibody against the 51-kDa subunit of complex I. Anal Biochem. 2003 Mar 15; 314(2):294-300. PMID: 12654316.
      View in: PubMed
    66. Rubinstein J, Walker J. ATP synthase from Saccharomyces cerevisiae: location of the OSCP subunit in the peripheral stalk region. J Mol Biol. 2002 Aug 23; 321(4):613-9. PMID: 12206777.
      View in: PubMed
    Rubinstein's networks
    Click the "See All" links for more information and interactive visualizations!
    Concepts Expand Description
    _
    Co-Authors Expand Description
    _
    Similar People Expand Description
    _
    Same Department Expand Description